Analytical and Bioanalytical Chemistry

, Volume 383, Issue 2, pp 282–290

Quantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flour

  • Philippe Corbisier
  • Stefanie Trapmann
  • David Gancberg
  • Liesbeth Hannes
  • Pierre Van Iwaarden
  • Gilbert Berben
  • Heinz Schimmel
  • Hendrik Emons
Original Paper

DOI: 10.1007/s00216-005-0013-x

Cite this article as:
Corbisier, P., Trapmann, S., Gancberg, D. et al. Anal Bioanal Chem (2005) 383: 282. doi:10.1007/s00216-005-0013-x

Abstract

Roundup Ready soybean powder has been subjected to different amounts of DNA fragmentation to assess the accuracy of real-time PCR on processed food. Certified reference material (CRM) containing 10 g kg−1 of Roundup Ready soybean (ERM-BF410d) prepared by a dry-mixing processing method was exposed to water at two temperatures, using three different mixing devices, or to baking temperature (250°C) for 30 min. The amount of DNA extracted from the different samples was quantified by fluorimetry. The amount of fragmentation of the extracted DNA was characterised by gel and capillary electrophoresis and the percentage of genetically modified (GM) soybean was determined by a double quantitative real-time PCR method. Measurement of the event GTS 40-3-2 (RUR) was possible in all the treated materials, because small amplicons were amplified. Correct RUR percentages could be measured for intact powders with little or no DNA fragmentation. For samples with a high level of DNA degradation, however, the accuracy of the measurement was found to depend on the method used for DNA extraction. Genomic DNA isolated by use of silica resin resulted in statistically significant overestimation of the amount of GM.

Keywords

Glycine max Genetically modified soybean (GMS) Polymerase chain reaction (PCR) Processed food DNA degradation 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Philippe Corbisier
    • 1
  • Stefanie Trapmann
    • 1
  • David Gancberg
    • 1
  • Liesbeth Hannes
    • 1
  • Pierre Van Iwaarden
    • 1
  • Gilbert Berben
    • 2
  • Heinz Schimmel
    • 1
  • Hendrik Emons
    • 1
  1. 1.Institute for Reference Materials and MeasurementsEuropean Commission, Joint Research CentreGeelBelgium
  2. 2.Quality Department of Agro-food ProductsWalloon Agricultural Research CentreGemblouxBelgium