Analytical and Bioanalytical Chemistry

, Volume 376, Issue 1, pp 26–32

Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas Putida

  • Rafael Pardo
  • Mar Herguedas
  • Enrique Barrado
  • Marisol Vega
Original Paper

DOI: 10.1007/s00216-003-1843-z

Cite this article as:
Pardo, R., Herguedas, M., Barrado, E. et al. Anal Bioanal Chem (2003) 376: 26. doi:10.1007/s00216-003-1843-z

Abstract

The accumulation of Cd(II), Cu(II), Pb(II) and Zn(II) at mg L−1 concentration levels by inactive freeze-dried biomass of Pseudomonas Putida has been investigated. These metals could be efficiently removed from diluted aqueous solutions. A contact time of 10 min was sufficient to reach equilibrium. The pH has a strong effect on metal biosorption and the optimal pH values were 6.0, 5.0–6.0, 6.0–6.5 and 7.0–7.5 for Cd(II), Cu(II), Pb(II) and Zn(II) respectively. Under these conditions there was 80% removal for all metals studied. The process of biosorption can be described by a Langmuir-type adsorption model. This model accounts for 98% of the data variance. The KA and qmax parameters for each metal are strongly correlated (at confidence levels greater than 98%) with the metal acidity, quantified by the constant of the corresponding M(OH)+ complex, thus confirming previous assertions by other authors.

Keywords

Heavy metals Biosorption P. Putida Biomass 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Rafael Pardo
    • 1
  • Mar Herguedas
    • 1
  • Enrique Barrado
    • 1
  • Marisol Vega
    • 1
  1. 1.Departamento de Química AnalíticaUniversidad de Valladolid, Facultad de CienciasValladolidSpain

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