Psychopharmacology

, Volume 138, Issue 1, pp 47–54

Elevated [3H]inositol 1,4,5-trisphosphate binding sites and expressed inositol 1,4,5-trisphosphate receptor protein level in platelets of depressed patients

Authors

  • Yogesh Dwivedi
    • Department of Psychiatry, Psychiatric Institute (M/C 912), University of Illinois at Chicago, 1601 West Taylor, Chicago, IL 60612, USA Fax: +1-312-433-8360
  • Philip G. Janicak
    • Department of Psychiatry, Psychiatric Institute (M/C 912), University of Illinois at Chicago, 1601 West Taylor, Chicago, IL 60612, USA Fax: +1-312-433-8360
  • G. N. Pandey
    • Department of Psychiatry, Psychiatric Institute (M/C 912), University of Illinois at Chicago, 1601 West Taylor, Chicago, IL 60612, USA Fax: +1-312-433-8360
ORIGINAL INVESTIGATION

DOI: 10.1007/s002130050644

Cite this article as:
Dwivedi, Y., Janicak, P. & Pandey, G. Psychopharmacology (1998) 138: 47. doi:10.1007/s002130050644

Abstract

Several reports suggest that serotonin2A (5HT2A) receptors and this receptor-mediated phosphatidyl inositol (PI) hydrolysis signal transduction system are altered in platelets of depressed patients. Inositol 1,4,5-trisphosphate (Ins[1,4,5]P3), an important component of the PI signaling system, plays a crucial role in various physiological processes by releasing Ca2+ from intracellular stores after binding with Ins(1,4,5)P3 receptors. To examine the role of Ins(1,4,5)P3 receptors in depression, we determined [3H]Ins(1,4,5)P3 binding sites and expressed protein levels of Ins(1,4,5)P3 receptors in platelets of depressed patients (n = 15) and normal control subjects (n = 17). We observed that the mean Bmax of [3H]Ins(1,4,5)P3 binding to Ins(1,4,5)P3 receptors was significantly higher in platelets of depressed subjects compared with normal control subjects, whereas there was no significant difference in KD between these two groups. The immuno-detectable expressed level of Ins(1,4,5)P3 receptor protein was also significantly increased in depressed patients in contrast to the levels of normal control subjects. Moreover, a significant correlation was observed in Bmax and the protein level of Ins(1,4,5)P3 receptors. The increase in the number of [3H]Ins(1,4,5)P3 binding sites in platelets of depressed subjects appears to be due to an increase in the amount of Ins(1,4,5)P3 receptor proteins. These results suggest that Ins(1,4,5)P3 receptors may be involved in the pathophysiology of depression.

Key words Inositol 145-trisphosphate receptorHuman plateletDepressionImmunolabeling
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© Springer-Verlag Berlin Heidelberg 1998