Psychopharmacology

, Volume 220, Issue 2, pp 319–330

Neurohypophyseal hormones manipulation modulate social and anxiety-related behavior in zebrafish

Authors

  • Daniela Braida
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
  • Andrea Donzelli
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
  • Roberta Martucci
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
  • Valeria Capurro
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
  • Marta Busnelli
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
    • CNR, Institute of Neuroscience
  • Bice Chini
    • CNR, Institute of Neuroscience
    • Department of Pharmacology, Chemotherapy and Medical ToxicologyUniversità degli Studi di Milano
    • CNR, Institute of Neuroscience
Original Investigation

DOI: 10.1007/s00213-011-2482-2

Cite this article as:
Braida, D., Donzelli, A., Martucci, R. et al. Psychopharmacology (2012) 220: 319. doi:10.1007/s00213-011-2482-2

Abstract

Rationale

Oxytocin (OT) and arginine-vasopressin (AVP) regulate social behavior in mammals. Zebrafish (Danio rerio) allows higher throughput and ease in studying human brain disorders.

Objectives

This study investigated in zebrafish the effect of non-mammalian homologs isotocin (IT) and vasotocin (AVT) in comparison with OT/AVP on social behavior and fear response to predator. The mechanism was studied using the most human selective OT and AVP receptor antagonists.

Methods

Zebrafish were injected i.m. with increasing doses (0.001–40 ng/kg) of the neuropeptides. DesGly-NH2-d(CH2)5-[d-Tyr2,Thr4]OVT) for OT receptor, SR 49059 for V1a subtype receptor, and SSR-149415 for V1b subtype receptor were injected i.m. 10 min before each agonist.

Results

All the peptides increased social preference and reduced fear to predator response in a dose-dependent manner interpolated by symmetrical parabolas. AVT/AVP were more potent to elicit anxiolytic than social effect while IT and OT were equally potent. All the antagonists dose-dependently inhibited both the effects induced by the neuropeptides. The ratio between the ED50 obtained for blocking the OT-induced effects on social preference and fear response to predator was very high only for desglyDTTyrOVT (160). SR49059 showed the highest ratio in blocking AVP-induced effects (807). The less selective antagonist appeared to be SSR149415.

Conclusions

For the first time, IT/AVT and OT/AVP were found to modulate in zebrafish, social behavior, unrelated to sex, and fear to predator response through at least two different receptors. Zebrafish is confirmed as a valid, reliable model to study deficit in social behavior characteristic of some psychiatric disorders.

Keywords

OxytocinVasopressinIsotocinVasotocinShoalingAnxietySocial behaviorSelective antagonist

Supplementary material

213_2011_2482_Fig6_ESM.jpg (61 kb)

Supplemental Fig. 1 Shoaling preference testing tank. P1 and P2 indicate preference areas for stimulus Nacre and WT, respectively. NP no preference area (according to Engeszer et al. 2008) (JPEG 61 kb)

Copyright information

© Springer-Verlag 2011