Psychopharmacology

, Volume 201, Issue 4, pp 471–481

Electrophysiological properties of dopamine neurons in the ventral tegmental area of Sardinian alcohol-preferring rats

  • Miriam Melis
  • Giuliano Pillolla
  • Simona Perra
  • Giancarlo Colombo
  • Anna Lisa Muntoni
  • Marco Pistis
Original Investigation

DOI: 10.1007/s00213-008-1309-2

Cite this article as:
Melis, M., Pillolla, G., Perra, S. et al. Psychopharmacology (2009) 201: 471. doi:10.1007/s00213-008-1309-2

Abstract

Rationale

Sardinian alcohol-preferring (sP) or -nonpreferring (sNP) rats are one of the few pairs of lines of rats selectively bred for their voluntary alcohol preference or aversion, respectively. Ventral tegmental area (VTA) dopamine (DA) neurons have long been implicated in many drug-related behaviors, including alcohol self-administration. However, the electrophysiological properties of these cells in sP and sNP rats remain unknown.

Objectives

This study was designed to examine the properties of posterior VTA DA neurons and to unveil functional differences between sP and sNP rats.

Materials and methods

The electrophysiological properties of DA cells were examined performing either single-cell extracellular recordings in anesthetized rats or whole-cell patch-clamp recordings in slices.

Results

Extracellular single-unit recordings revealed an increased spontaneous activity in sP rats. However, a corresponding difference was not found in vitro. Moreover, DA cells of sP and sNP rats showed similar intrinsic properties, suggesting changes at synaptic level. Therefore, inhibitory- and excitatory-mediated currents were studied. A decreased probability of GABA release was found in sP rats. Additionally, sP rats showed a reduced depolarization-induced suppression of inhibition, which is an endocannabinoid-mediated form of short-term plasticity. Additionally, the effect of cannabinoid-type 1 (CB1) receptor agonist WIN55,212-2 on GABAA IPSCs was smaller in sP rats, suggesting either a reduced number or functionality of CB1 receptors in the VTA.

Conclusions

Our findings suggest that both decreased GABA release and endocannabinoid transmission in the VTA play a role in the increased impulse activity of DA cells and, ultimately, in alcohol preference displayed by sP rats.

Keywords

AlcoholDopamine neuronsElectrophysiologyEndocannabinoidsSardinian alcohol-preferring (sP) ratsVentral tegmental areaCannabinoids

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Miriam Melis
    • 1
    • 2
  • Giuliano Pillolla
    • 1
    • 2
  • Simona Perra
    • 1
    • 2
    • 4
  • Giancarlo Colombo
    • 3
  • Anna Lisa Muntoni
    • 2
    • 3
  • Marco Pistis
    • 1
    • 2
  1. 1.“B.B. Brodie” Department of NeuroscienceUniversity of Cagliari, Cittadella Universitaria di MonserratoMonserratoItaly
  2. 2.Centre of Excellence “Neurobiology of Addiction”University of CagliariMonserratoItaly
  3. 3.C.N.R. Institute of NeuroscienceMonserratoItaly
  4. 4.Waggoner Center for Alcohol and Addiction Research, Section of Neurobiology and Institute for NeuroscienceUniversity of TexasAustinUSA