Psychopharmacology

, Volume 179, Issue 1, pp 189–197

NMDA or AMPA/kainate receptor blockade prevents acquisition of conditioned place preference induced by D2/3 dopamine receptor stimulation in rats

Authors

  • Anna-Maria Biondo
    • Department of SociologyUniversity of Alberta
  • Robert L. H. Clements
    • W.G. Dewhurst Laboratory, Department of Psychiatry, 1E7.44 Mackenzie Health Sciences CentreUniversity of Alberta
    • Centre for NeuroscienceUniversity of Alberta
  • David J. Hayes
    • W.G. Dewhurst Laboratory, Department of Psychiatry, 1E7.44 Mackenzie Health Sciences CentreUniversity of Alberta
    • Centre for NeuroscienceUniversity of Alberta
  • Brendan Eshpeter
    • Department of PsychologyUniversity of Alberta
    • W.G. Dewhurst Laboratory, Department of Psychiatry, 1E7.44 Mackenzie Health Sciences CentreUniversity of Alberta
    • Centre for NeuroscienceUniversity of Alberta
Original Investigation

DOI: 10.1007/s00213-005-2201-y

Cite this article as:
Biondo, A., Clements, R.L.H., Hayes, D.J. et al. Psychopharmacology (2005) 179: 189. doi:10.1007/s00213-005-2201-y

Abstract

Rationale

Recent experiments from this laboratory demonstrated synergistic effects of AMPA/kainate receptor blockade and D2/3 dopamine (DA) receptor stimulation on brain stimulation reward and locomotor activity.

Objectives

Using place conditioning, this study explored further the interaction between DA and glutamate (Glu) using the N-methyl-d-aspartate (NMDA) receptor antagonist MK-801, the AMPA/kainate receptor antagonist NBQX, and the D2/3 DA receptor agonist 7-OH-DPAT.

Methods

Effects of these compounds, alone and combined, were measured in male Sprague–Dawley rats using an unbiased two-compartment place conditioning procedure.

Results

7-OH-DPAT (0.03–5.0 mg kg−1, s.c.) administered immediately prior to conditioning was ineffective; when administered 15 min prior to conditioning, only the highest dose (5.0 mg kg−1, s.c.) induced conditioned place preference (CPP). Acquisition of 7-OH-DPAT-induced CPP was blocked by MK-801 (0.06 or 0.13 mg kg−1, i.p.) or NBQX (0.5 μg) microinjected into the nucleus accumbens (NAS) shell subregion. Intra-NAS shell administration of 7-OH-DPAT (5.0 μg) or NBQX (0.5 μg), alone or combined, failed to induce place conditioning, and this lack of effect was not due to state dependency. Administration of MK-801 or 7-OH-DPAT (5.0 mg kg−1) during the conditioning phase acutely increased horizontal activity, but neither compound, alone or combined, induced conditioned locomotor effects.

Conclusions

Acquisition of place conditioning induced by systemic administration of 7-OH-DPAT is blocked by systemic NMDA receptor antagonism by MK-801 or by the AMPA/kainate receptor antagonist NBQX microinjected into the NAS shell subregion.

Keywords

7-OH-DPATAMPA/kainateDopamineGlutamateLocomotor activityMK-801NMDAPlace conditioningPlace preferenceReward

Copyright information

© Springer-Verlag 2005