Psychopharmacology

, Volume 180, Issue 3, pp 391–398

Serotonin transport kinetics correlated between human platelets and brain synaptosomes

  • Jeffrey L. Rausch
  • Maria E. Johnson
  • Junqing Li
  • Julian Hutcheson
  • Benjamin M. Carr
  • Katina M. Corley
  • Amanda B. Gowans
  • Joseph Smith
Original Investigation

DOI: 10.1007/s00213-005-2178-6

Cite this article as:
Rausch, J.L., Johnson, M.E., Li, J. et al. Psychopharmacology (2005) 180: 391. doi:10.1007/s00213-005-2178-6

Abstract

Rationale

Blood platelets have been used extensively as a model system for investigating the role of the serotonin transporter (SERT) in various psychiatric disorders, especially depression. However, to date, it is not known whether platelet serotonin (5-HT) transport would be related to that in brain.

Objectives

We examined 5-HT transport kinetics simultaneously in human blood platelets and human cortical brain synaptosomes to determine whether they were correlated.

Methods

Blood platelets and synaptosomes were obtained from 25 patients undergoing epileptic surgery. Synaptosomes were obtained from normal margins of surgical neuropathology specimens of anterotemporal cortex.

Results

Platelet SERT Vmax was significantly correlated with brain SERT Vmax on linear regression (r=0.58, p<0.005), after controlling for the confounding effects of gender (t=−2.4, p=0.025) and time of day (t=2.1, p<0.05). Consistent with previous observations, there was a negative correlation between the maximum velocity (Vmax) of platelet 5-HT transport and pO2 (r=−0.52, p<0.01). Females had a significantly higher pO2 than males (F=4.9, p<0.05). After accounting for gender differences, addition of pO2 did not add further strength to the regression, given the aforementioned gender differences in pO2. The correlation between unadjusted values for platelet vs brain SERT Vmax was r=0.3, p=0.06.

Conclusions

These results suggest that a relationship may exist between 5-HT transport in platelets and cortical synaptosomes, when appropriate controls for confounding factors are employed.

Keywords

Serotonin transport (SERT) Uptake Human brain Platelet Depression Epilepsy 5-HT 5-HTT pO2 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jeffrey L. Rausch
    • 1
    • 2
  • Maria E. Johnson
    • 2
  • Junqing Li
    • 1
  • Julian Hutcheson
    • 1
    • 2
  • Benjamin M. Carr
    • 2
  • Katina M. Corley
    • 2
  • Amanda B. Gowans
    • 2
  • Joseph Smith
    • 3
  1. 1.Veterans Administration Hospital, AugustaAugustaUSA
  2. 2.Department of Psychiatry and Health Behavior, School of MedicineThe Medical College of GeorgiaAugustaUSA
  3. 3.Department of Surgery, School of MedicineThe Medical College of GeorgiaAugustaUSA

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