Original Investigation

Psychopharmacology

, Volume 181, Issue 3, pp 496-503

Hallucinogen-like actions of 2,5-dimethoxy-4-(n)-propylthiophenethylamine (2C-T-7) in mice and rats

  • William E. FantegrossiAffiliated withDivision of Neuroscience, Yerkes National Primate Research Center, Emory UniversityDepartment of Pharmacology, University of Michigan Medical School Email author 
  • , Andrew W. HarringtonAffiliated withDepartment of Pharmacology, University of Michigan Medical School
  • , Justin R. EcklerAffiliated withDepartment of Pharmacology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo
  • , Sadia ArshadAffiliated withDepartment of Pharmacology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo
  • , Richard A. RabinAffiliated withDepartment of Pharmacology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo
  • , Jerrold C. WinterAffiliated withDepartment of Pharmacology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo
  • , Andrew CoopAffiliated withDepartment of Pharmaceutical Sciences, University of Maryland School of Pharmacy
  • , Kenner C. RiceAffiliated withLaboratory of Medicinal Chemistry, NIDDK, National Institutes of Health
  • , James H. WoodsAffiliated withDepartment of Pharmacology, University of Michigan Medical School

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Abstract

Rationale

Few studies have examined the effects of 2,5-dimethoxy-4-(n)-propylthiophenethylamine (2C-T-7) in vivo.

Objectives

2C-T-7 was tested in a drug-elicited head twitch assay in mice and in several drug discrimination assays in rats; 2C-T-7 was compared to the phenylisopropylamine hallucinogen R(−)-1-(2,5-dimethoxy-4-methylphenyl)-2aminopropane (DOM) in both assays, with or without pretreatment with the selective 5-HT2A antagonist (+)-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenylethyl)]-4-piperidine-methanol (M100907). Finally, the affinity of 2C-T-7 for three distinct 5-HT receptors was determined in rat brain.

Methods

Drug-elicited head twitches were quantified for 10 min following administration of various doses of either 2C-T-7 or R(−)-DOM, with and without pretreatments of 0.01 mg/kg M100907. In rats trained to discriminate lysergic acid diethylamide (LSD), 2C-T-7 and R(−)-DOM were tested for generalization. In further studies, rats were trained to discriminate 2C-T-7 from saline, then challenged with 0.05 mg/kg M100907. In competition binding studies, the affinity of 2C-T-7 was assessed at 5-HT2A receptors, 5-HT1A receptors, and 5-HT2C receptors.

Results

2C-T-7 and R(−)-DOM induced similar head twitch responses in the mouse that were antagonized by M100907. In the rat, 2C-T-7 produced an intermediate degree of generalization (75%) to the LSD cue and served as a discriminative stimulus; these interoceptive effects were attenuated by M100907. Finally, 2C-T-7 had nanomolar affinity for 5-HT2A and 5-HT2C receptors and lower affinity for 5-HT1A receptors.

Conclusions

2C-T-7 is effective in two rodent models of 5-HT2 agonist activity and has affinity at receptors relevant to hallucinogen effects. The effectiveness with which M100907 antagonizes the behavioral actions of 2C-T-7 strongly suggests that the 5-HT2A receptor is an important site of action for this compound.

Keywords

2C-T-7 Hallucinogens Phenethylamines Drug discrimination Head twitch response 5-HT2A receptor Mice Rats