Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 362, Issue 1, pp 60–67

Characterization of histamine H3 receptors in mouse brain using the H3 antagonist [125I]iodophenpropit

Authors

  • Frank P. Jansen
    • Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
  • Takatoshi Mochizuki
    • Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan
  • Kazutaka Maeyama
    • Department of Pharmacology, Ehime University School of Medicine, Ehime 791-02, Japan
  • Rob Leurs
    • Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
  • Hendrik Timmerman
    • Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
Original Article

DOI: 10.1007/s002100000227

Cite this article as:
Jansen, F., Mochizuki, T., Maeyama, K. et al. Naunyn-Schmied Arch Pharmacol (2000) 362: 60. doi:10.1007/s002100000227

Abstract.

We have characterized the binding of the histamine H3 receptor antagonist [125I]iodophenpropit to mouse brain. [125I]Iodophenpropit saturably bound to mouse brain membranes with a pKd-value of 9.31±0.04 nM and a receptor binding density of 290±8 fmol per mg protein. Saturation binding analysis revealed binding of [125I]iodophenpropit to a single class of sites, showing linear Scatchard plots and Hill coefficients not different from unity (nH=0.98±0.02). At a concentration of 0.25 nM [125I]iodophenpropit, specific binding represented about 75% of the total binding. Competition binding curves for H3 receptor antagonists were fitted best to a one-site model, showing pKi-values in general accordance with the pA2-values obtained in mouse cerebral cortex. Displacement of [125I]iodophenpropit by the H3 receptor agonists (R)-α-methylhistamine, immepip, imetit and histamine were fitted best to a two-site model. Competition binding curves of (R)-α-methylhistamine showed a rightward shift upon incubation with GTPγS (10 µM), indicating the involvement of G-proteins in H3 agonist binding. In contrast, competition binding curves of the antagonists iodophenpropit, thioperamide and burimamide were not affected by GTPγS (10 µM). Autoradiographic experiments showed that [125I]iodophenpropit binding sites were heterogeneously distributed, similarly to the distribution of histamine H3 receptors reported in rat brain. Highest densities were observed in the cerebral cortex, the striatum, the nucleus accumbens, the globus pallidus and the substantia nigra.

In conclusion, we have demonstrated that in mouse brain, [125I]iodophenpropit selectively binds to histamine H3 receptors. We also observed that the mouse brain H3 receptors labelled by [125I]iodophenpropit displayed binding characteristics and a distribution similar to rat brain.

Histamine H3 receptors [125I]Iodophenpropit Receptor binding study Mouse brain Autoradiographic distribution

Copyright information

© Springer-Verlag 2000