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Green tea extract with polyethylene glycol-3350 reduces body weight and improves glucose tolerance in db/db and high-fat diet mice

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Abstract

Green tea extract (GTE) is regarded to be effective against obesity and type 2 diabetes, but definitive evidences have not been proven. Based on the assumption that the gallated catechins (GCs) in GTE attenuate intestinal glucose and lipid absorption, while enhancing insulin resistance when GCs are present in the circulation through inhibiting cellular glucose uptake in various tissues, this study attempted to block the intestinal absorption of GCs and prolong their residence time in the lumen. We then observed whether GTE containing the nonabsorbable GCs could ameliorate body weight (BW) gain and glucose intolerance in db/db and high-fat diet mice. Inhibition of the intestinal absorption of GCs was accomplished by co-administering the nontoxic polymer polyethylene glycol-3350 (PEG). C57BLKS/J db/db and high-fat diet C57BL/6 mice were treated for 4 weeks with drugs as follows: GTE, PEG, GTE + PEG, voglibose, or pioglitazone. GTE mixed with meals did not have any ameliorating effects on BW gain and glucose intolerance. However, the administration of GTE plus PEG significantly reduced BW gain, insulin resistance, and glucose intolerance, without affecting food intake and appetite. The effect was comparable to the effects of an α-glucosidase inhibitor and a peroxisome proliferator-activated receptor-γ/α agonist. These results indicate that prolonging the action of GCs of GTE in the intestinal lumen and blocking their entry into the circulation may allow GTE to be used as a prevention and treatment for both obesity and obesity-induced type 2 diabetes.

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Acknowledgments

This work was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (grant number 110135-3). Miss Sun-Joo Kim in Keimyung University School of Medicine provided valued technical assistance and care of the animals.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Physiology and Endocrinology, Keimyung University School of Medicine, 1095 Dalgubeoldae-Ro, Dalseo-Gu, Daegu, 704-701, South Korea

    Jae-Hyung Park, Ho-Chan Cho, Ki-Cheor Bae, Seung-Soon Im, Jae-Hoon Bae & Dae-Kyu Song

  2. Department of Pathology, Keimyung University School of Medicine, 1095 Dalgubeoldae-Ro, Dalseo-Gu, Daegu, 704-701, South Korea

    Sang Pyo Kim

  3. Department of Physiology, Yeongnam University School of Medicine, 317-1, Daemyung-Dong, Nam-Gu, Daegu, 705-717, South Korea

    Yoon Jung Choi & Yong Woon Kim

  4. GCB R&D and Clinical Trial Center Co., Ltd., 906-5 Eeu-Dong, Youngdong-Gu, Suwon, Gyeonggi-do, South Korea

    Shinbyoung Ahn

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  1. Jae-Hyung Park
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Correspondence to Dae-Kyu Song.

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Park, JH., Choi, Y.J., Kim, Y.W. et al. Green tea extract with polyethylene glycol-3350 reduces body weight and improves glucose tolerance in db/db and high-fat diet mice. Naunyn-Schmiedeberg's Arch Pharmacol 386, 733–745 (2013). https://doi.org/10.1007/s00210-013-0869-9

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