, Volume 386, Issue 2, pp 97-105
Date: 20 Nov 2012

Interaction of D3 preferring agonist (−)-N 6-(2-(4-(biphenyl-4-yl)piperazin-1-yl)ethyl)-N 6-propyl-4,5,6,7-tetrahydrobenzo[d]thiazole-2,6-diamine (D-264) with cloned human D2L, D2S, and D3 receptors: potent stimulation of mitogen-activated protein kinases and G protein-coupled inward rectifier potassium channels

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Abstract

This study aims to determine the effect of the novel D3 dopamine receptor agonist, D-264, on activation of D3 and D2 dopamine receptor signal transduction pathways and cell proliferation. AtT-20 neuroendocrine cells stably expressing human D2S, D2L, and D3 dopamine receptors were treated with D-264 and the coupling of the receptors to mitogen-activated protein kinase (MAPK) and G protein-coupled inward rectifier potassium (GIRK) channels was determined using Western blotting and whole-cell voltage clamp recording, respectively. D-264 potently activated MAPK signaling pathway coupled to D2S, D2L, and D3 dopamine receptors. The activation of MAPK was more pronounced than the reference agonist quinpirole and was longer lasting. D-264 also activated GIRK channels coupled to D2S, D2L, and D3 receptors. In addition, D-264 dose-dependently induced cell proliferation in AtT-D2L and AtT-D3 cells. These results indicate that D-264 robustly activates GIRK channels and MAPK coupled to D2 and D3 dopamine receptors in AtT-20 cells. D-264 is also a potent inducer of cell proliferation.