Naunyn-Schmiedeberg's Archives of Pharmacology

, 381:73

Protective effect of selaginellin on glutamate-induced cytotoxicity and apoptosis in differentiated PC12 cells

  • Chen-Jing Wang
  • Chang-Ping Hu
  • Kang-Ping Xu
  • Qiong Yuan
  • Fu-Shuang Li
  • Hui Zou
  • Gui-Shan Tan
  • Yuan-Jian Li
ORIGINAL ARTICLE

DOI: 10.1007/s00210-009-0470-4

Cite this article as:
Wang, CJ., Hu, CP., Xu, KP. et al. Naunyn-Schmied Arch Pharmacol (2010) 381: 73. doi:10.1007/s00210-009-0470-4

Abstract

l-glutamate plays a key role in neuronal cell death associated with many neurodegenerative conditions such as cerebral ischemia, hypoxia, Alzheimer's, Huntington’s, and Parkinson’s diseases. Selaginellin, a component extracted from Saussurea pulvinata (Hook.et Grev.) Maximo, was assessed for its ability to protect rat pheochromocytoma (PC12) cells against oxidative toxicity induced by glutamate. The differentiated PC12 cells were pretreated with various concentrations (10−7, 3 × 10−7, or 10−6 M) of selaginellin for 1 h prior to exposure to l-glutamate. Selaginellin was shown to protect PC12 cells against glutamate toxicity, as determined by characteristic morphological features, lactate dehydrogenase release and cell viability, and apoptosis as evaluated by Hoechst 33342 staining assay and caspase-3 activity. In addition, the increase in levels of reactive oxygen species and decrease in klotho gene expression induced by glutamate were significantly reversed by selaginellin. Our study suggests that selaginellin has a neuroprotective effect against l-glutamate-induced neurotoxicity through mechanisms related to anti-oxidation and anti-apoptosis via scavenging reactive oxygen species and up-regulating the expression of klotho gene.

Keywords

SelaginellinGlutamateReactive oxygen speciesOxidative stressPC12klotho gene

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Chen-Jing Wang
    • 1
  • Chang-Ping Hu
    • 1
  • Kang-Ping Xu
    • 2
  • Qiong Yuan
    • 1
  • Fu-Shuang Li
    • 2
  • Hui Zou
    • 2
  • Gui-Shan Tan
    • 2
  • Yuan-Jian Li
    • 1
  1. 1.Department of Pharmacology, School of Pharmaceutical SciencesCentral South UniversityChangshaChina
  2. 2.Chemistry Section, Department of Medicinal Chemistry, School of Pharmaceutical SciencesCentral South UniversityChangshaChina