Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 373, Issue 1, pp 60–70

β2-Adrenergic stimulation is involved in the contractile dysfunction of the stunned heart

Original Article

DOI: 10.1007/s00210-006-0045-6

Cite this article as:
Vittone, L., Said, M. & Mattiazzi, A. Naunyn Schmied Arch Pharmacol (2006) 373: 60. doi:10.1007/s00210-006-0045-6


Endogenous catecholamines released during myocardial ischemia have been considered both to aggravate cell injury and exacerbate arrhythmias and to exert a protective action on the post-ischemic contractile function. The present work was addressed to look for evidence to explain this controversy. The effects of cardiac catecholamine depletion and of α- and β-adrenoceptor (AR) blockade on the post-ischemic contractile dysfunction, as well as its possible relationship with cardiac oxidative stress, were studied in isolated and perfused rat hearts submitted to 20 min of ischemia and 30 min of reperfusion (stunning). Catecholamine depletion improves the contractile recovery in the stunned heart. This mechanical effect was associated with decreased levels of lipid peroxidation. A similar enhancement of the contractile function during reperfusion was detected after the simultaneous blockade of α1- and β-ARs with prazosin plus propranolol. To ascertain which specific AR pathway was involved in the effects of catecholamines on the stunned heart, selective AR blockers, prazosin (α1-blocker), atenolol (β1-blocker), ICI 118,551 (β2-blocker) and selective inhibitors of Gi-PI3K pathway (pertussis toxin and wortmannin) were alternatively combined. The results indicate that catecholamines released during ischemia exert a dual action on the contractile behavior of the stunned heart: a deleterious effect, related to the activation of the β2-AR-Gi-PI3K-pathway, which was counteracted by a beneficial effect, triggered by the stimulation of α1-AR. Neither the depression nor the enhancement of the post-ischemic contractile recovery were related with the increase in ROS formation induced by endogenous catecholamines.


Stunned heart Endogenous catecholamines β2-adrenergic contractile effect 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Leticia Vittone
    • 1
  • Matilde Said
    • 1
  • Alicia Mattiazzi
    • 1
  1. 1.Centro de Investigaciones Cardiovasculares, Facultad de Ciencias MédicasUniversidad Nacional de La PlataLa PlataArgentina