Archives of Toxicology

, Volume 71, Issue 12, pp 746–750

Ethoxyresorufin-O-deethylase (EROD) inducing potencies of planar chlorinated aromatic hydrocarbons in primary cultures of hepatocytes from different developmental stages of the chicken

  • Albertus T. C. Bosveld
  • Sean W. Kennedy
  • Willem Seinen
  • Martin van den Berg
METABOLIC ACTIVATION/INACTIVATION

DOI: 10.1007/s002040050456

Cite this article as:
Bosveld, A., Kennedy, S., Seinen, W. et al. Arch Toxicol (1997) 71: 746. doi:10.1007/s002040050456

Abstract

In vitro induction of ethoxyresorufin O-deethylase (EROD) activity in cell cultures is an extensively validated tool for measuring overall potencies of mixtures of halogenated aromatic hydrocarbons (HAHs) in samples from the abiotic or biotic environment. For risk assessment with special attention to effects in wild birds, an assay was developed that makes use of chicken embryo hepatocytes. However, it was questioned whether compound-specific responses are consistent at the various developmental stages. The results of our present study show that there are considerable differences between early and late embryonal and post-hatching stages. The induction of EROD was measured in primary chicken hepatocyte cultures. The cells were isolated at day 14 and day 19 of embryonal development and at day 1 post hatching. Hepatocytes were exposed in vitro to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF), 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126, IUPAC nomenclature) and 2,3′,4,4′,5-pentachlorobiphenyl (PCB 118). The respective compounds were chosen as representives for dioxins, furans, non-ortho PCBs, and mono-ortho PCBs. These groups of chemicals have been identified as environmental contaminants with major dioxin-like effects that are mediated by a common receptor, the arylhydrocarbon (Ah) receptor. At all developmental stages, TCDF was more potent than TCDD. Relative potencies (RP = EC50tcdd/EC50hah) decreased in the order TCDF<TCDD< PCB 126<PCB 118. Depending on the developmental stage, TCDF was 1.2 to 3.4 times more potent than TCDD. PCB 126 was equipotent or less potent by a factor of 3 than TCDD. PCB 118 was 100 to 300 times less potent than TCDD. Both the mean effective concentration (EC50) and the maximum EROD activity (Ymax) of all compounds were lower in hepatocyte cultures from 14-day-old embryos than those from 19-day-old embryos or 1-day-old hatchlings. RPs were comparable in 19-day-old embryos and in hatchlings, but significantly different in 14-day-old embryos.

Key words 2378-Tetrachlorodibenzo-p-dioxinTCDD2378-TetrachlorodibenzofuranTCDF33′44′5-Pentachlorobiphenyl2344′5-PentachlorobiphenylPCBIn vitroRelative potency

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Albertus T. C. Bosveld
    • 1
  • Sean W. Kennedy
    • 2
  • Willem Seinen
    • 3
  • Martin van den Berg
    • 3
  1. 1.Institute for Forestry and Nature Research/IBN-DLO, Dept. Ecotoxicology, P.O. Box 23, NL-6700 AA Wageningen, The NetherlandsNL
  2. 2.Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, 100 Gamelin Blvd., Hull, Quebec K1A 0H3, CanadaCA
  3. 3.Research Institute of Toxicology, Utrecht University, P.O. Box 80 176, NL-3508 TD Utrecht, The NetherlandsNL