Archives of Toxicology

, Volume 88, Issue 2, pp 391–401

Circulating acylcarnitines as biomarkers of mitochondrial dysfunction after acetaminophen overdose in mice and humans

  • Mitchell R. McGill
  • Feng Li
  • Matthew R. Sharpe
  • C. David Williams
  • Steven C. Curry
  • Xiaochao Ma
  • Hartmut Jaeschke
Molecular Toxicology

DOI: 10.1007/s00204-013-1118-1

Cite this article as:
McGill, M.R., Li, F., Sharpe, M.R. et al. Arch Toxicol (2014) 88: 391. doi:10.1007/s00204-013-1118-1

Abstract

Acetaminophen (APAP) is a widely used analgesic. However, APAP overdose is hepatotoxic and is the primary cause of acute liver failure in the developed world. The mechanism of APAP-induced liver injury begins with protein binding and involves mitochondrial dysfunction and oxidative stress. Recent efforts to discover blood biomarkers of mitochondrial damage have identified increased plasma glutamate dehydrogenase activity and mitochondrial DNA concentration in APAP overdose patients. However, a problem with these markers is that they are too large to be released from cells without cell death or loss of membrane integrity. Metabolomic studies are more likely to reveal biomarkers that are useful at early time points, before injury begins. Similar to earlier work, our metabolomic studies revealed that acylcarnitines are elevated in serum from mice after treatment with toxic doses of APAP. Importantly, a comparison with furosemide demonstrated that increased serum acylcarnitines are specific for mitochondrial dysfunction. However, when we measured these compounds in plasma from humans with liver injury after APAP overdose, we could not detect any significant differences from control groups. Further experiments with mice showed that N-acetylcysteine, the antidote for APAP overdose in humans, can reduce acylcarnitine levels in serum. Altogether, our data do not support the clinical measurement of acylcarnitines in blood after APAP overdose due to the standard N-acetylcysteine treatment in patients, but strongly suggest that acylcarnitines would be useful mechanistic biomarkers in other forms of liver injury involving mitochondrial dysfunction.

Keywords

Acetaminophen toxicity Biomarkers Mitochondria Acylcarnitines 

Abbreviations

ALT

Alanine aminotransferase

APAP

Acetaminophen

CACT

Carnitine-acylcarnitine translocase

CPT

Carnitine palmitoyltransferase

GDH

Glutamate dehydrogenase

JNK

c-Jun N-terminal kinase

MLK3

Mixed lineage kinase 3

MPT

Mitochondrial membrane permeability transition

mtDNA

Mitochondrial DNA

NAC

N-acetylcysteine

NAPQI

N-acetyl-p-benzoquinone imine

OPLS-DA

Orthogonal projection to latent structures-discriminant analysis

PCA

Principal component analysis

ROS

Reactive oxygen species

Supplementary material

204_2013_1118_MOESM1_ESM.docx (560 kb)
Supplementary material 1 (DOCX 560 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mitchell R. McGill
    • 1
  • Feng Li
    • 1
  • Matthew R. Sharpe
    • 2
  • C. David Williams
    • 1
  • Steven C. Curry
    • 3
    • 4
  • Xiaochao Ma
    • 1
  • Hartmut Jaeschke
    • 1
  1. 1.Department of Pharmacology, Toxicology, and TherapeuticsUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Internal MedicineUniversity of Kansas HospitalKansas CityUSA
  3. 3.Department of Medical ToxicologyBanner Good Samaritan Medical CenterPhoenixUSA
  4. 4.Department of Medicine, Center for Toxicology and Pharmacology Education and ResearchUniversity of Arizona College of MedicinePhoenixUSA

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