Archives of Toxicology

, Volume 86, Issue 12, pp 1851–1859

Fine PM induce airway MUC5AC expression through the autocrine effect of amphiregulin

  • Stéphanie Val
  • Esther Belade
  • Isabelle George
  • Jorge Boczkowski
  • Armelle Baeza-Squiban
Molecular Toxicology

DOI: 10.1007/s00204-012-0903-6

Cite this article as:
Val, S., Belade, E., George, I. et al. Arch Toxicol (2012) 86: 1851. doi:10.1007/s00204-012-0903-6

Abstract

Particulate pollution is suspected to contribute to obstructive lung diseases characterized by chronic inflammation, mucus hypersecretion and bronchial remodeling. Our aim was to study the effect of real-world particulate matter (PM) on the expression of a mucin, MUC5AC, focusing on the role of the epidermal growth factor receptor (EGFR) pathway. MUC5AC induction was studied in vivo in mice trachea and in vitro in human bronchial epithelial cells (HBEC) exposed to urban fine PM. Fine PM were able to induce MUC5AC mRNA in mice trachea after 48 h of exposure (50 μg PM/mouse), and MUC5AC mRNA and protein in HBEC after 24 h of exposure (from 5 μg PM/cm2). It was associated with the increased expression of amphiregulin (AREG), an EGFR ligand. Experiments with conditioned media (media from PM-treated cells) demonstrated the involvement of AREG on MUC5AC induction as MUC5AC induction by media from PM-treated cells was prevented in the presence of either EGFR- or AREG-neutralizing antibodies. The effect of an inhibitor of a metalloprotease involved in the AREG shedding confirmed the autocrine loop made by AREG leading to MUC5AC induction by fine PM. We also demonstrated that IL-8 pro-inflammatory cytokine induction was dependent on the same autocrine mechanisms. We demonstrate for the first time that MUC5AC expression and production is increased by short-term exposure to fine PM through an autocrine effect of AREG. Our study provides mechanistic explanations to the exacerbation of obstructive lung diseases induced by particulate pollution characterized by mucus hypersecretion and chronic inflammation.

Keywords

Atmospheric particleEGFR pathwayLungMucinPro-inflammatory cytokine

Supplementary material

204_2012_903_MOESM1_ESM.pdf (30 kb)
Supplementary material 1 (PDF 30 kb)
204_2012_903_MOESM2_ESM.pdf (90 kb)
Supplementary material 2 (PDF 90 kb)
204_2012_903_MOESM3_ESM.pdf (89 kb)
Supplementary material 3 (PDF 89 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Stéphanie Val
    • 1
  • Esther Belade
    • 2
    • 3
  • Isabelle George
    • 1
  • Jorge Boczkowski
    • 2
    • 3
    • 4
    • 5
  • Armelle Baeza-Squiban
    • 1
  1. 1.Laboratory of Molecular and Cellular Responses to Xenobiotics, Unit of Functional and Adaptive Biology (BFA) EAC CNRS 4413University Paris Diderot, Sorbonne Paris CitéParisFrance
  2. 2.INSERM U955 Equipe 4CréteilFrance
  3. 3.Faculté de MédecineUniversité Paris EstCréteilFrance
  4. 4.Centre Hospitalier Intercommunal, Service de Pneumologie et Pathologie ProfessionnelleCréteilFrance
  5. 5.AP-HP, Hôpital Henri Mondor, Service de Physiologie Explorations FonctionnellesCréteilFrance