Archives of Toxicology

, Volume 85, Issue 6, pp 607–612

Simple and quick method for whole-liver decellularization: a novel in vitro three-dimensional bioengineering tool?

  • Joery De Kock
  • Liesbeth Ceelen
  • Ward De Spiegelaere
  • Christophe Casteleyn
  • Paul Claes
  • Tamara Vanhaecke
  • Vera Rogiers
Toxicokinetics and Metabolism

DOI: 10.1007/s00204-011-0706-1

Cite this article as:
De Kock, J., Ceelen, L., De Spiegelaere, W. et al. Arch Toxicol (2011) 85: 607. doi:10.1007/s00204-011-0706-1

Abstract

Proof of principle of organ reengineering through the development of a transplantable recellularized liver graft was published recently. As the decellularization time of the rat liver took 72 h, loss of some key matrix proteins seemed inevitable. Here, we describe the development of a three-dimensional naturally derived liver scaffold with an intact microvascular system that is capable of withstanding fluid flows in the three hepatic circular systems and that is obtained within 60 min. For this purpose, whole rat livers were sequentially perfused with a selection of mild tensioactive substances to remove the cellular components while preserving the major extracellular matrix proteins, including laminin, collagen I, collagen IV, and fibronectin. In addition, we could show the presence of extracellular matrix–bound growth factor islets, important for cell engraftment, migration, proliferation, and differentiation. This easy to prepare scaffold could represent a remarkable tool in the bioengineering of complex three-dimensional in vitro systems for advanced preclinical drug development.

Keywords

Liver matrixOrgan decellularizationBiological liver scaffoldThree-dimensional scaffold

Abbreviations

DLM

Decellularized liver matrix

ECM

Extracellular matrix

KHB

Krebs-Henseleit buffer

PBS

Phosphate-buffered saline

SEM

Scanning electron microscopy

SDS

Sodium dodecyl sulfate

3D

Three-dimensional

VEGF

Vascular endothelial growth factor

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Joery De Kock
    • 1
  • Liesbeth Ceelen
    • 1
  • Ward De Spiegelaere
    • 2
  • Christophe Casteleyn
    • 2
  • Paul Claes
    • 1
  • Tamara Vanhaecke
    • 1
  • Vera Rogiers
    • 1
  1. 1.Department of Toxicology, Center for Pharmaceutical ResearchVrije Universiteit Brussel (VUB)BrusselsBelgium
  2. 2.Department of Morphology, Faculty of Veterinary MedicineUniversiteit Gent (UGent)MerelbekeBelgium