Archives of Toxicology

, Volume 83, Issue 4, pp 357–362

Vitamin D metabolism impairment in the rat’s offspring following maternal exposure to 137cesium

  • E. Tissandie
  • Y. Guéguen
  • J. M. A. Lobaccaro
  • L. Grandcolas
  • S. Grison
  • J. Aigueperse
  • M. Souidi
Organ Toxicity and Mechanisms

DOI: 10.1007/s00204-008-0351-5

Cite this article as:
Tissandie, E., Guéguen, Y., Lobaccaro, J.M.A. et al. Arch Toxicol (2009) 83: 357. doi:10.1007/s00204-008-0351-5

Abstract

Previous works clearly showed that chronic contamination by 137cesium alters vitamin D metabolism. Since children are known to be a high-risk group for vitamin D metabolism disorders, effects of 137Cs on vitamin D biosynthetic pathway were investigated in newborn rats. The experiments were performed in 21-day-old male offspring of dams exposed to 137Cs in their drinking water at a dose of 6,500 Bq/l (150 Bq/rat/day) during the lactation period. Significant modifications of blood calcium (−7%, P < 0.05), phosphate (+80%, P < 0.01) and osteocalcin (−25%, P < 0.05) levels were observed in contaminated offspring, associated with an increase of blood vitamin D3 (+25%, P < 0.01). Besides, decreased expression levels of cyp2r1 and cyp27b1 (−26 and −39%, respectively, P < 0.01) were measured in liver and kidney suggesting a physiological adaptation in response to the rise in vitamin D level. Expressions of vdr, ecac1, cabp-d28k, ecac2 and cabp-9k involved in renal and intestinal calcium transport were unaffected. Altogether, these data show that early exposure to post-accidental doses of 137Cs induces the alteration of vitamin D metabolism, associated with a dysregulation of mineral homeostasis.

Keywords

137CesiumChronic contaminationCytochrome P450Vitamin D3Maternal exposure

Abbreviations

1,25(OH)2D3

1α,25-Dihydroxyvitamin D3

25(OH)D3

25-Hydroxyvitamin D3

137Cs

Cesium

ALT

Alanine amino-transferase

AST

Aspartate amino-transferase

CaBP-D

Calbindin-D

CYP

Cytochrome P450

ECaC

Epithelial Ca2+ channel

PTH

Parathyroid hormone

RXR

Retinoid X receptor

VDR

Vitamin D receptor

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • E. Tissandie
    • 1
  • Y. Guéguen
    • 1
  • J. M. A. Lobaccaro
    • 2
  • L. Grandcolas
    • 1
  • S. Grison
    • 1
  • J. Aigueperse
    • 1
  • M. Souidi
    • 1
  1. 1.Radiological Protection and Human Health Division, Radiobiology and Epidemiology Department, Laboratory of Experimental ToxicologyInstitute for Radiological Protection and Nuclear SafetyFontenay-aux-Roses CedexFrance
  2. 2.Compared Physiology and Molecular EndocrinologyUMR Université Blaise Pascal-CNRS 6547Aubière CedexFrance