Archives of Toxicology

, 83:81

β-Glucan extracted from the medicinal mushroom Agaricus blazei prevents the genotoxic effects of benzo[a]pyrene in the human hepatoma cell line HepG2

Authors

  • José Pedro Friedmann Angeli
    • Laboratório de Genética Toxicológica, Departamento de Biologia GeralUniversidade Estadual de Londrina
  • Lúcia Regina Ribeiro
    • Programa de Pós-Graduação em Patologia, Faculdade de MedicinaUNESP
    • Programa de Pós-Graduação em Biologia Celular e Molecular, Depto. de BiologiaUNESP
  • Marilanda Ferreira Bellini
    • Laboratório de Genética Toxicológica, Departamento de Biologia GeralUniversidade Estadual de Londrina
    • Laboratório de Genética Toxicológica, Departamento de Biologia GeralUniversidade Estadual de Londrina
Genotoxicity and Carcinogenicity

DOI: 10.1007/s00204-008-0319-5

Cite this article as:
Angeli, J.P.F., Ribeiro, L.R., Bellini, M.F. et al. Arch Toxicol (2009) 83: 81. doi:10.1007/s00204-008-0319-5

Abstract

The mushroom Agaricus blazei is studied for its nutraceutical potential and as a medicinal supplement. The aim of the present study was to investigate the chemoprotective effect of β-glucan extracted from the mushroom A. blazei against DNA damage induced by benzo[a]pyrene (B[a]P), using the comet assay (genotoxicity) and micronucleus assay with cytokinesis block (mutagenicity) in a human hepatoma cell line (HepG2). To elucidate the possible β-glucan mechanism of action, desmutagenesis or bioantimutagenesis types, three treatment protocols were tested: simultaneous, pre-treatment, and presimultaneous. The results showed that β-glucan does not exert genotoxic or mutagenic effect, but that it does protect against DNA damage caused by B[a]P in every protocol tested. The data suggest that β-glucan acts through binding to B[a]P or the capture of free radicals produced during its activation. On the other hand, the pre-treatment results also suggest the possibility that β-glucan modulates cell metabolism.

Keywords

β-GlucanComet assayMicronucleus assayHepG2AntigenotoxicityAntimutagenicity

Copyright information

© Springer-Verlag 2008