Archives of Toxicology

, Volume 82, Issue 6, pp 363–370

Resveratrol attenuates cisplatin-induced nephrotoxicity in rats

  • Cátia Lira Do Amaral
  • Heloísa Della Coletta Francescato
  • Terezila Machado Coimbra
  • Roberto Silva Costa
  • Joana D’arc Castania Darin
  • Lusânia Maria Greggi Antunes
  • Maria De Lourdes Pires Bianchi
Organ Toxicity and Mechanisms

DOI: 10.1007/s00204-007-0262-x

Cite this article as:
Do Amaral, C.L., Francescato, H.D.C., Coimbra, T.M. et al. Arch Toxicol (2008) 82: 363. doi:10.1007/s00204-007-0262-x

Abstract

Cisplatin is an antitumor drug widely used in the treatment of many malignant tumors. However, the most common adverse effect, nephrotoxicity, limits the use of this drug in many cancer patients. Resveratrol is a phytoalexin that presents highly efficient protection in experimental nephrotoxicity models. This study evaluated the effect of resveratrol on cisplatin-induced kidney damage. Male Wistar rats were treated with resveratrol (25 mg/kg b.w., i.p.) before the administration of cisplatin (5 mg/kg b.w., i.p.) and killed 2 or 5 days later. Blood and urine samples were collected and the kidneys were removed. Rats from the cisplatin group showed acute tubular cell necrosis and increased immunostaining for ED1 (macrophages/monocytes) and T-lymphocytes in the renal cortex and outer medulla when compared with the control group. These alterations were less intense in animals pre-treated with resveratrol. Moreover, indicators of renal injury such as increased serum creatinine levels, urinary volume and urinary protein caused by the administration of cisplatin, were also significantly reduced with resveratrol. Increased lipid peroxidation and glutathione depletion in tissue were attenuated by resveratrol. In conclusion, resveratrol attenuated the cisplatin-induced structural and functional renal changes by reducing free radicals and inhibiting inflammatory cell infiltrates.

Keywords

Cisplatin Resveratrol Nephrotoxicity Macrophage T-lymphocyte 

Abbreviations

ATN

Acute tubular necrosis

cDDP

Cisplatin

GSH

Reduced glutathione

MDA

Malondialdehyde

Res

Resveratrol

ROS

Reactive oxygen species

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Cátia Lira Do Amaral
    • 1
  • Heloísa Della Coletta Francescato
    • 2
  • Terezila Machado Coimbra
    • 2
  • Roberto Silva Costa
    • 3
  • Joana D’arc Castania Darin
    • 1
  • Lusânia Maria Greggi Antunes
    • 1
  • Maria De Lourdes Pires Bianchi
    • 1
  1. 1.Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Faculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São PauloRibeirao PretoBrazil
  2. 2.Departamento de Fisiologia, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloRibeirao PretoBrazil
  3. 3.Departamento de Patologia, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloRibeirao PretoBrazil

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