Archives of Toxicology

, Volume 77, Issue 7, pp 418–423

Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase

  • Ruei-Ming Chen
  • Gong-Jhe Wu
  • Yi-Ting Tai
  • Wei-Zen Sun
  • Yi-Ling Lin
  • Wen-Chi Jean
  • Ta-Liang Chen
Molecular Toxicology

DOI: 10.1007/s00204-003-0453-z

Cite this article as:
Chen, RM., Wu, GJ., Tai, YT. et al. Arch Toxicol (2003) 77: 418. doi:10.1007/s00204-003-0453-z

Abstract

Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 µM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination of propofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 µM led to significant cell death (P<0.05). The levels of nitrite, an oxidative product of nitric oxide, were increased in lipopolysaccharide-treated macrophages in a concentration-dependent manner (P<0.01), while propofol could concentration-dependently decrease the lipopolysaccharide-enhanced nitrite levels (P<0.01). Immunoblotting analysis revealed that lipopolysaccharide increased the protein level of inducible nitric oxide synthase (iNOS). The co-treatment of propofol and lipopolysaccharide significantly reduced this lipopolysaccharide-induced iNOS protein (357±49×103 versus 92±6×103 arbitrary units, P<0.01). Analysis by reverse transcriptase-polymerase chain reaction showed that lipopolysaccharide induced mRNA of iNOS, but that the inductive effect was inhibited by propofol (95±7×102 versus 30±4×102 arbitrary units, P<0.01). This study has demonstrated that propofol, at therapeutic concentrations, could suppress nitric oxide biosynthesis by inhibiting iNOS expression in lipopolysaccharide-activated macrophages. The mechanism of suppression was at a pretranslational level.

Keywords

PropofolMacrophagesLipopolysaccharideInducible nitric oxide synthaseNitric oxide

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Ruei-Ming Chen
    • 1
  • Gong-Jhe Wu
    • 2
  • Yi-Ting Tai
    • 1
  • Wei-Zen Sun
    • 3
  • Yi-Ling Lin
    • 1
  • Wen-Chi Jean
    • 1
  • Ta-Liang Chen
    • 1
  1. 1.Department of Anesthesiology,Graduate Institute of Medical Science, College of Medicine, Wan-Fang HospitalTaipei Medical UniversityTaipeiTaiwan
  2. 2.Department of AnesthesiologyShin Kong Wu Ho-Su Memorial HospitalTaipeiTaiwan
  3. 3.Department of Anesthesiology, College of Medicine and HospitalNational Taiwan UniversityTaipeiTaiwan