Original paper

Archives of Microbiology

, Volume 171, Issue 3, pp 183-188

Ferribacterium limneticum, gen. nov., sp. nov., an Fe(III)-reducing microorganism isolated from mining-impacted freshwater lake sediments

  • David E. CummingsAffiliated withDepartment of Biological Sciences, University of Idaho, Moscow, ID 83844–3051 USA e-mail: rrose@uidaho.edu Tel. +1-208-885-7764; Fax +1-208-885-7905
  • , Frank Caccavo Jr.Affiliated withDepartment of Microbiology, University of New Hampshire, Durham, NH 03824 USA
  • , Stefan SpringAffiliated withLehrstuhl für Mikrobiologie, Technische Universität München, D-80290 Munich, Germany
  • , R. F. RosenzweigAffiliated withDepartment of Biological Sciences, University of Idaho, Moscow, ID 83844–3051 USA e-mail: rrose@uidaho.edu Tel. +1-208-885-7764; Fax +1-208-885-7905

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Abstract

A dissimilatory Fe(III)-reducing bacterium was isolated from mining-impacted lake sediments and designated strain CdA-1. The strain was isolated from a 4-month enrichment culture with acetate and Fe(III)-oxyhydroxide. Strain CdA-1 is a motile, obligately anaerobic rod, capable of coupling the oxidation of acetate and other organic acids to the reduction of ferric iron. Fe(III) reduction was not observed using methanol, ethanol, isopropanol, propionate, succinate, fumarate, H2, citrate, glucose, or phenol as potential electron donors. With acetate as an electron donor, strain CdA-1 also grew by reducing nitrate or fumarate. Growth was not observed with acetate as electron donor and O2, sulfoxyanions, nitrite, trimethylamine N-oxide, Mn(IV), As(V), or Se(VI) as potential terminal electron acceptors. Comparative 16 S rRNA gene sequence analyses show strain CdA-1 to be most closely related (93.6% sequence similarity) to Rhodocyclus tenuis. However, R. tenuis did not grow heterotrophically by Fe(III) reduction, nor did strain CdA-1 grow photrophically. We propose that strain CdA-1 represents a new genus and species, Ferribacterium limneticum. Strain CdA-1 represents the first dissimilatory Fe(III) reducer in the β subclass of Proteobacteria, as well as the first Fe(III) reducer isolated from mine wastes.

Key wordsFerribacterium limneticum Dissimilatory iron reduction Mine wastes Lake Coeur d’Alene Idaho