Archives of Microbiology

, Volume 171, Issue 1, pp 66–68

Biosynthesis of cobalamin in Salmonella typhimurium: transformation of riboflavin into the 5,6-dimethylbenzimidazole moiety

Authors

  • Bettina Keck
    • Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstrasse 30, D-70599 Stuttgart, Germany e-mail: b-c-nutr@uni-hohenheim.de Tel. +49-711-459-2284; Fax +49-711-459-2283
  • Michael Munder
    • Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstrasse 30, D-70599 Stuttgart, Germany e-mail: b-c-nutr@uni-hohenheim.de Tel. +49-711-459-2284; Fax +49-711-459-2283
  • P. Renz
    • Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstrasse 30, D-70599 Stuttgart, Germany e-mail: b-c-nutr@uni-hohenheim.de Tel. +49-711-459-2284; Fax +49-711-459-2283
Short communication

DOI: 10.1007/s002030050679

Cite this article as:
Keck, B., Munder, M. & Renz, P. Arch Microbiol (1998) 171: 66. doi:10.1007/s002030050679

Abstract

In order to elucidate the biosynthesis of the base moiety of cobalamin in Salmonella typhimurium LT2, this organism was grown in the presence of [1′-14C]riboflavin. The vitamin B12 isolated was 14C-labeled. It was shown by chemical degradation that the 14C-label was exclusively localized in carbon atom 2 of the 5,6-dimethylbenzimidazole moiety. This demonstrated the precursor function of riboflavin in the biosynthesis of 5,6-dimethylbenzimidazole in S. typhimurium.

Key words 56-DimethylbenzimidazoleRiboflavinSalmonella typhimuriumVitamin B12

Copyright information

© Springer-Verlag Berlin Heidelberg 1998