Archives of Microbiology

, Volume 168, Issue 5, pp 380–388

Dissimilatory arsenate and sulfate reduction in Desulfotomaculum auripigmentum sp. nov.

  • Dianne K. Newman
  • Erin K. Kennedy
  • John D. Coates
  • Dianne Ahmann
  • Debra J. Ellis
  • Derek R. Lovley
  • François M. M. Morel
Original paper

DOI: 10.1007/s002030050512

Cite this article as:
Newman, D., Kennedy, E., Coates, J. et al. Arch Microbiol (1997) 168: 380. doi:10.1007/s002030050512

Abstract

A newly discovered arsenate-reducing bacterium, strain OREX-4, differed significantly from strains MIT-13 and SES-3, the previously described arsenate-reducing isolates, which grew on nitrate but not on sulfate. In contrast, strain OREX-4 did not respire nitrate but grew on lactate, with either arsenate or sulfate serving as the electron acceptor, and even preferred arsenate. Both arsenate and sulfate reduction were inhibited by molybdate. Strain OREX-4, a gram-positive bacterium with a hexagonal S-layer on its cell wall, metabolized compounds commonly used by sulfate reducers. Scorodite (FeAsO42· H2O) an arsenate-containing mineral, provided micromolar concentrations of arsenate that supported cell growth. Physiologically and phylogenetically, strain OREX-4 was far-removed from strains MIT-13 and SES-3: strain OREX-4 grew on different electron donors and electron acceptors, and fell within the gram-positive group of the Bacteria, whereas MIT-13 and SES-3 fell together in the ɛ-subdivision of the Proteobacteria. Together, these results suggest that organisms spread among diverse bacterial phyla can use arsenate as a terminal electron acceptor, and that dissimilatory arsenate reduction might occur in the sulfidogenic zone at arsenate concentrations of environmental interest. 16S rRNA sequence analysis indicated that strain OREX-4 is a new species of the genus Desulfotomaculum, and accordingly, the name Desulfotomaculum auripigmentum is proposed.

Key wordsDesulfotomaculum Arsenate reduction Sulfate reduction 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Dianne K. Newman
    • 1
  • Erin K. Kennedy
    • 2
  • John D. Coates
    • 3
  • Dianne Ahmann
    • 4
  • Debra J. Ellis
    • 5
  • Derek R. Lovley
    • 6
  • François M. M. Morel
    • 2
  1. 1.Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USAUS
  2. 2.Department of Geosciences, Guyot Hall, Princeton University, Princeton, NJ 08544, USA Tel. +1-609-258-1052; Fax +1-609-258-5242 e-mail: dianne@phoenix.princeton.eduUS
  3. 3.Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USAUS
  4. 4.School of the Environment, Duke University, Durham, NC 27704, USAUS
  5. 5.American Type Culture Collection, 12031 Parklawn Drive, Rockville, MD 20852, USAUS
  6. 6.Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USAUS

Personalised recommendations