Archives of Microbiology

, Volume 167, Issue 1, pp 46–53

Methylosulfonomonas methylovora gen. nov., sp. nov., and Marinosulfonomonas methylotropha gen. nov., sp. nov.: novel methylotrophs able to grow on methanesulfonic acid

  • Andrew J. Holmes
  • D. P. Kelly
  • Simon C. Baker
  • A. S. Thompson
  • Paolo De Marco
  • Elizabeth M. Kenna
  • J. Colin Murrell
Original paper

DOI: 10.1007/s002030050415

Cite this article as:
Holmes, A., Kelly, D., Baker, S. et al. Arch Microbiol (1997) 167: 46. doi:10.1007/s002030050415

Abstract

Two novel genera of restricted facultative methylotrophs are described; both Methylosulfonomonas and Marinosulfonomonas are unique in being able to grow on methanesulfonic acid as their sole source of carbon and energy. Five identical strains of Methylosulfonomonas were isolated from diverse soil samples in England and were shown to differ in their morphology, physiology, DNA base composition, molecular genetics, and 16S rDNA sequences from the two marine strains of Marinosulfonomonas, which were isolated from British coastal waters. The marine strains were almost indistinguishable from each other and are considered to be strains of one species. Type species of each genus have been identified and named Methylosulfonomonas methylovora (strain M2) and Marinosulfonomonas methylotropha (strain PSCH4). Phylogenetic analysis using 16S rDNA sequencing places both genera in the α-Proteobacteria. Methylosulfonomonas is a discrete lineage within the α-2 subgroup and is not related closely to any other known bacterial genus. The Marinosulfonomonas strains form a monophyletic cluster in the α-3 subgroup of the Proteobacteria with Roseobacter spp. and some other partially characterized marine bacteria, but they are distinct from these at the genus level. This work shows that the isolation of bacteria with a unique biochemical character, the ability to grow on methanesulfonic acid as energy and carbon substrate, has resulted in the identification of two novel genera of methylotrophs that are unrelated to any other extant methylotroph genera.

Key wordsMethylosulfonomonas methylovoraMarinosulfonomonas methylotrophaMethanesulfonicacid16S rRNA phylogeny16S rDNA sequencesProteobacteria

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Andrew J. Holmes
    • 2
  • D. P. Kelly
    • 1
  • Simon C. Baker
    • 3
  • A. S. Thompson
    • 2
  • Paolo De Marco
    • 2
  • Elizabeth M. Kenna
    • 2
  • J. Colin Murrell
    • 2
  1. 1.Institute of Education, University of Warwick, Coventry CV4 7AL, England Tel. + 44-1203-523891; Fax + 44-1203-524110 e-mail: d.p.kelly@warwick.ac.ukGB
  2. 2.Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, EnglandGB
  3. 3.Department of Biochemistry, University of Oxford, Oxford OX1 3QU, EnglandGB