Archives of Microbiology

, Volume 198, Issue 4, pp 315–324

The emergence of epitheliocystis in the upper Rhone region: evidence for Chlamydiae in wild and farmed salmonid populations

  • Maricruz Guevara Soto
  • Beatriz Vidondo
  • Lloyd Vaughan
  • Helena M. B. Seth-Smith
  • Lisbeth Nufer
  • Helmut Segner
  • Jean-Francois Rubin
  • Heike Schmidt-Posthaus
Original Paper

DOI: 10.1007/s00203-016-1192-x

Cite this article as:
Guevara Soto, M., Vidondo, B., Vaughan, L. et al. Arch Microbiol (2016) 198: 315. doi:10.1007/s00203-016-1192-x

Abstract

We present the first study comparing epitheliocystis in a wild and farmed salmonid in Europe. Sampling three tributaries to the Lake Geneva, including one from headwaters to river mouth, revealed an unequal distribution of epitheliocystis in brown trout (Salmotrutta). When evaluated histologically and comparing sites grouped as wild versus farm, the probability of finding infected trout is higher on farms. In contrast, the infection intensities, as estimated by the number of cysts per gill arch, were higher on average and showed maximum values in the wild trout. Sequence analysis showed the most common epitheliocystis agents were Candidatus Piscichlamydia salmonis, all clustering into a single clade, whereas Candidatus Clavichlamydia salmonicola sequences cluster in two closely related subspecies, of which one was mostly found in farmed fish and the other exclusively in wild brown trout, indicating that farms are unlikely to be the source of infections in wild trout. A detailed morphological analysis of cysts using transmission electron microscopy revealed unique features illustrating the wide divergence existing between Ca. P. salmonis and Ca. C. salmonicola within the phylum Chlamydiae.

Keywords

Brown trout Candidatus Piscichlamydia salmonis Candidatus Clavichlamydia salmonicola River Farm 

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Maricruz Guevara Soto
    • 1
  • Beatriz Vidondo
    • 2
  • Lloyd Vaughan
    • 3
  • Helena M. B. Seth-Smith
    • 4
  • Lisbeth Nufer
    • 3
  • Helmut Segner
    • 1
  • Jean-Francois Rubin
    • 5
  • Heike Schmidt-Posthaus
    • 1
  1. 1.Centre of Fish and Wildlife Health, Department of Infectious Diseases and PathobiologyUniversity of BernBernSwitzerland
  2. 2.Institute of Veterinary Public HealthUniversity of BernBernSwitzerland
  3. 3.Institute of Veterinary PathologyUniversity of ZurichZurichSwitzerland
  4. 4.Functional Genomics Center ZurichUniversity of ZurichZurichSwitzerland
  5. 5.Fondation de la Maison de la RivièreTolochenazSwitzerland