Archives of Microbiology

, Volume 195, Issue 1, pp 75–80

PknE, a serine/threonine protein kinase from Mycobacterium tuberculosis has a role in adaptive responses

Authors

  • Dinesh Kumar
    • Department of ImmunologyNational Institute for Research in Tuberculosis (Indian Council of Medical Research)
  • Kannan Palaniyandi
    • Department of ImmunologyNational Institute for Research in Tuberculosis (Indian Council of Medical Research)
  • Vijay K. Challu
    • National Tuberculosis InstituteDirectorate General of Health Services
  • Prahlad Kumar
    • National Tuberculosis InstituteDirectorate General of Health Services
    • Department of ImmunologyNational Institute for Research in Tuberculosis (Indian Council of Medical Research)
Short Communication

DOI: 10.1007/s00203-012-0848-4

Cite this article as:
Kumar, D., Palaniyandi, K., Challu, V.K. et al. Arch Microbiol (2013) 195: 75. doi:10.1007/s00203-012-0848-4

Abstract

Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfactant and cell wall–damaging agents) and anti-tuberculosis drugs. ΔpknE had a defective growth in pH 7.0 and lysozyme (a cell wall–damaging agent) with better survival in pH 5.5, SDS and kanamycin (a second-line anti-tuberculosis drug). Furthermore, ΔpknE was reduced in cell size during growth in liquid media and exhibited hypervirulence in a guinea pig model of infection. In conclusion, our data suggest that pknE plays a role in adaptive response of M. tuberculosis regulating cellular integrity and survival.

Keywords

Mycobacterium tuberculosisSTPKPknE and stress

Copyright information

© Springer-Verlag Berlin Heidelberg 2012