Archives of Microbiology

, Volume 194, Issue 11, pp 901–907

Acetatifactor muris gen. nov., sp. nov., a novel bacterium isolated from the intestine of an obese mouse

Authors

  • Nora Pfeiffer
    • Gastrointestinal MicrobiologyGerman Institute of Human Nutrition Potsdam-Rehbrücke
  • Charles Desmarchelier
    • Molecular Nutrition Unit, ZIEL, Research Centre for Nutrition and Food SciencesTechnische Universität München
    • Faculté de MédecineINSERM, UMR 1062 « Nutrition, Obesity and Risk of Thrombosis »/INRA, UMR1260/Université d’Aix-Marseille
  • Michael Blaut
    • Gastrointestinal MicrobiologyGerman Institute of Human Nutrition Potsdam-Rehbrücke
  • Hannelore Daniel
    • Molecular Nutrition Unit, ZIEL, Research Centre for Nutrition and Food SciencesTechnische Universität München
  • Dirk Haller
    • Biofunctionality, ZIEL, Research Centre for Nutrition and Food SciencesTechnische Universität München
    • Biofunctionality, ZIEL, Research Centre for Nutrition and Food SciencesTechnische Universität München
    • Young Research Group Intestinal MicrobiomeTU München, ZIEL, BFLM
Original Paper

DOI: 10.1007/s00203-012-0822-1

Cite this article as:
Pfeiffer, N., Desmarchelier, C., Blaut, M. et al. Arch Microbiol (2012) 194: 901. doi:10.1007/s00203-012-0822-1

Abstract

We used selective agar media for culturing bacteria from the caecum of mice fed a high calorie diet. In addition to the isolation of Enterobacteriaceae growing on a medium containing cholesterol and bile salts, we focused on the characterization of strain CT-m2T, which, based on 16S rDNA analysis, did not appear to correspond to any currently described organisms. The isolate belongs to the Clostridium cluster XIV and is most closely related to members of the Lachnospiraceae, including the genera Anaerostipes, Blautia, Butyrivibrio, Clostridium, Coprococcus, Eubacterium, Robinsoniella, Roseburia, Ruminococcus and Syntrophococcus (≤90 % similarity). Strain CT-m2T is a non-motile Gram-positive rod that does not form spores and has a G + C content of DNA of 48.5 %. Cells grow under strictly anoxic conditions (100 % N2) and produce acetate and butyrate after growth in reduced WCA broth. In contrast to related species, the new bacterium does not metabolize glucose and is positive for phenylalanine arylamidase, and its major cellular fatty acid is C14:0. Based on phylogenetic and phenotypic studies, the isolate merits recognition as a member of a novel genus and species, for which the name Acetatifactor muris is proposed. The type strain is CT-m2T (= DSM 23669T = ATCC BAA-2170T).

Keywords

Mouse intestinal microbiotaDiet-induced obesityFirmicutesClostridiaAcetatifactor muris

Supplementary material

203_2012_822_MOESM1_ESM.doc (951 kb)
Supplementary material 1 (DOC 951 kb)

Copyright information

© Springer-Verlag 2012