Archives of Microbiology

, Volume 193, Issue 1, pp 45–52

Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA

Original Paper

DOI: 10.1007/s00203-010-0639-8

Cite this article as:
Osburn, M.R. & Amend, J.P. Arch Microbiol (2011) 193: 45. doi:10.1007/s00203-010-0639-8

Abstract

A hyperthermophilic heterotrophic archaeon (strain WB1) was isolated from a thermal pool in the Washburn hot spring group of Yellowstone National Park, USA. WB1 is a coccus, 0.6–1.2 μm in diameter, with a tetragonal S-layer, vacuoles, and occasional stalk-like protrusions. Growth is optimal at 84°C (range 64–93°C), pH 5–6 (range 3.5–8.5), and <1 g/l NaCl (range 0–4.6 g/l NaCl). Tests of metabolic properties show the isolate to be a strict anaerobe that ferments complex organic substrates. Phylogenetic analysis of the 16S rRNA gene sequence places WB1 in a clade of previously uncultured Desulfurococcaceae and shows it to have ≤96% 16S rRNA sequence identity to Desulfurococcus mobilis, Staphylothermus marinus, Staphylothermus hellenicus, and Sulfophobococcus zilligii. The 16S rRNA gene contains a large insertion similar to homing endonuclease introns reported in Thermoproteus and Pyrobaculum species. Growth is unaffected by the presence of S0 or SO42−, thereby differentiating the isolate from its closest relatives. Based on phylogenetic and physiological differences, it is proposed that isolate WB1 represents the type strain of a novel genus and species within the Desulfurococcaceae, Thermogladius shockii gen. nov., sp. nov. (RIKEN = JCM-16579, ATCC = BAA-1607, Genbank 16S rRNA gene = EU183120).

Keywords

Yellowstone national park Desulfurococcaceae Novel species Thermophile 

Supplementary material

203_2010_639_MOESM1_ESM.tif (3.5 mb)
Fig. S1: Additional phylogenetic tree of the selected member of the Desulfurococcales constructed using a neighbor joining method. GenBank accession numbers are given in parentheses and superscript T indicates type strain. (TIFF 3,587 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of Earth and Planetary SciencesWashington UniversitySt. LouisUSA
  2. 2.Division of Geological and Planetary Sciences MC170-25California Institute of TechnologyPasadenaUSA