Archives of Microbiology

, Volume 192, Issue 11, pp 909–918

Non-enzymatic roles for the URE2 glutathione S-transferase in the response of Saccharomyces cerevisiae to arsenic

  • Tatina T. Todorova
  • Anna V. Kujumdzieva
  • Stéphane Vuilleumier
Original Paper

DOI: 10.1007/s00203-010-0614-4

Cite this article as:
Todorova, T.T., Kujumdzieva, A.V. & Vuilleumier, S. Arch Microbiol (2010) 192: 909. doi:10.1007/s00203-010-0614-4

Abstract

The response of Saccharomyces cerevisiae to arsenic involves a large ensemble of genes, many of which are associated with glutathione-related metabolism. The role of the glutathione S-transferase (GST) product of the URE2 gene involved in resistance of S. cerevisiae to a broad range of heavy metals was investigated. Glutathione peroxidase activity, previously reported for the Ure2p protein, was unaffected in cell-free extracts of an ure2Δ mutant of S. cerevisiae. Glutathione levels in the ure2Δ mutant were lowered about threefold compared to the isogenic wild-type strain but, as in the wild-type strain, increased 2–2.5-fold upon addition of either arsenate (AsV) or arsenite (AsIII). However, lack of URE2 specifically caused sensitivity to arsenite but not to arsenate. The protective role of URE2 against arsenite depended solely on the GST-encoding 3′-end portion of the gene. The nitrogen source used for growth was suggested to be an important determinant of arsenite toxicity, in keeping with non-enzymatic roles of the URE2 gene product in GATA-type regulation.

Keywords

Ure2Glutathione S-transferaseGATA regulationArsenic detoxification

Abbreviations

GSTs

Glutathione S-transferases

NCR

Nitrogen catabolite repression

GPx

Glutathione peroxidase

Gdh1

NADPH-dependent glutamate dehydrogenase

Gln1

Glutamine synthetase

Gdh2

NAD+-dependent glutamate dehydrogenase

Supplementary material

203_2010_614_MOESM1_ESM.doc (48 kb)
Supplementary material 1 (DOC 47 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Tatina T. Todorova
    • 1
    • 2
  • Anna V. Kujumdzieva
    • 1
  • Stéphane Vuilleumier
    • 2
  1. 1.Faculty of Biology, Department of General and Applied MicrobiologySofia University “St. Kliment Ohridski”SofiaBulgaria
  2. 2.Université de StrasbourgStrasbourg CédexFrance