Archives of Microbiology

, Volume 191, Issue 9, pp 721–733

Yeast protein phosphatases Ptp2p and Msg5p are involved in G1–S transition, CLN2 transcription, and vacuole morphogenesis

  • Hermansyah
  • Minetaka Sugiyama
  • Yoshinobu Kaneko
  • Satoshi Harashima
Original Paper

DOI: 10.1007/s00203-009-0498-3

Cite this article as:
Hermansyah, Sugiyama, M., Kaneko, Y. et al. Arch Microbiol (2009) 191: 721. doi:10.1007/s00203-009-0498-3

Abstract

We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca2+ sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca2+-induced stress response in a redundant manner. To gain insight into the molecular mechanism causing calcium sensitivity of the ∆ptp2msg5 double disruptant, we performed fluorescence-activated cell sorting analysis and found a delayed G1 phase. This delayed G1 was consistent with the defect in bud emergence, and reduced CLN2 transcription upon addition of CaCl2. We also found that Slt2p is hyper-phosphorylated in the Δptp2 Δmsg5 double disruptant and that the vacuole of the Δptp2 Δmsg5 double disruptant is fragmented even in the absence of Ca2+. These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.

Keywords

Saccharomyces cerevisiaeProtein phosphatasePTP2MSG5Calcium sensitivityDelayed G1

Abbreviations

PPase

Protein phosphatase

MAPK

Mitogen-activated protein kinase

Cas

Calcium sensitive

CWI

Cell wall integrity

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Hermansyah
    • 1
  • Minetaka Sugiyama
    • 1
  • Yoshinobu Kaneko
    • 1
  • Satoshi Harashima
    • 1
  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuita, OsakaJapan