Archives of Microbiology

, Volume 191, Issue 8, pp 675–679

Amiodarone inhibits multiple drug resistance in yeast Saccharomyces cerevisiae

Authors

    • A.N. Belozersky Institute of Physico-Chemical BiologyMoscow State University
  • Tatiana N. Krivonosova
    • A.N. Belozersky Institute of Physico-Chemical BiologyMoscow State University
  • Olga V. Markova
    • A.N. Belozersky Institute of Physico-Chemical BiologyMoscow State University
  • Fedor F. Severin
    • A.N. Belozersky Institute of Physico-Chemical BiologyMoscow State University
Short Communication

DOI: 10.1007/s00203-009-0493-8

Cite this article as:
Knorre, D.A., Krivonosova, T.N., Markova, O.V. et al. Arch Microbiol (2009) 191: 675. doi:10.1007/s00203-009-0493-8

Abstract

Amiodarone is a widely used antiarrhythmic drug. There is also evidence that amiodarone decreases multidrug resistance in human cell lines. In this paper, we have shown that amiodarone has similar effect on yeast, Saccharomyces cerevisiae, decreasing multiple drug resistance. Amiodarone stimulates the accumulation of ethidium bromide by inhibiting its efflux from the cells. The effect of amiodarone is much stronger on wild-type cells compared to the mutant with inactivated ABC-transporters. Interestingly, the action of amiodarone is additive with the one of chloroquine, a known inhibitor of ABC-transporters. We speculate that these findings could help in the development of antifungal drug mixes.

Keywords

YeastAmiodaronePleiotropic drug resistanceMultidrug resistance

Abbreviations

ABC

ATP-binding cassette

MDR

Multiple drug resitance

EB

Ethidium bromide

C12-TPP

Dodecyltriphenylphosphonium

Copyright information

© Springer-Verlag 2009