Archives of Microbiology

, Volume 191, Issue 6, pp 501–507

Characterization of chsA, a new gene controlling the chemotactic response in Azospirillum brasilense Sp7

  • Ricardo Carreño-López
  • Araceli Sánchez
  • Nohemí Camargo
  • Claudine Elmerich
  • Beatriz Eugenia Baca
Original Paper

DOI: 10.1007/s00203-009-0475-x

Cite this article as:
Carreño-López, R., Sánchez, A., Camargo, N. et al. Arch Microbiol (2009) 191: 501. doi:10.1007/s00203-009-0475-x

Abstract

We report, here, the characterization of a mutant strain of Azospirillum brasilense Sp7 impaired in surface motility and chemotactic response. Presence of flagella in the mutant strain was confirmed by western blot analysis, using antisera raised against the polar and lateral flagellins, and by electron microscopy. Genetic complementation and nucleotide sequencing led to the identification of a new gene, named chsA. The deduced translation product, ChsA protein, contained a PAS sensory domain and an EAL domain. As ChsA displayed characteristic signaling protein architecture, it is thought that this protein is a component of the signaling pathway controlling chemotaxis in Azospirillum.

Keywords

Azospirillum brasilenseChsA signaling proteinChemotactic response

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Ricardo Carreño-López
    • 1
  • Araceli Sánchez
    • 1
  • Nohemí Camargo
    • 1
  • Claudine Elmerich
    • 2
  • Beatriz Eugenia Baca
    • 1
  1. 1.Centro de Investigaciones en Ciencias MicrobiológicasBenemérita Universidad Autónoma de PueblaPueblaMexico
  2. 2.Département de MicrobiologieBMGE, Institut PasteurParisFrance