Archives of Microbiology

, Volume 188, Issue 2, pp 175–184

Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance

  • Iwona Wojda
  • Jan-Paul Bebelman
  • Teresa Jakubowicz
  • Marco Siderius
Original Paper

DOI: 10.1007/s00203-007-0234-9

Cite this article as:
Wojda, I., Bebelman, JP., Jakubowicz, T. et al. Arch Microbiol (2007) 188: 175. doi:10.1007/s00203-007-0234-9
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Abstract

A Saccharomyces cerevisiae strain in which the GPP1 and GPP2 genes, both encoding glycerol-3-phosphate phosphatase isoforms, are deleted, displays both osmo- and thermosensitive (ts) phenotypes. We isolated genes involved in cell wall maintenance as multicopy suppressors of the gpp1gpp2 ts phenotype. We found that the gpp1gpp2 strain is hypersensitive to cell wall stress such as treatment with β-1,3-glucanase containing cocktail Zymolyase and chitin-binding dye Calcofluor-white (CFW). Sensitivity to Zymolyase was rescued by overexpression of SSD1, while CFW sensitivity was rescued by SSD1, FLO8 and WSC3—genes isolated as multicopy suppressors of the gpp1gpp2 ts phenotype. Some of the isolated suppressor genes (SSD1, FLO8) also rescued the lytic phenotype of slt2 deletion strain. Additionally, the sensitivity to CFW was reduced when the cells were supplied with glycerol. Both growth on glycerol-based medium and overexpression of SSD1, FLO8 or WSC3 had additive suppressing effect on CFW sensitivity of the gpp1gpp2 mutant strain. We also confirmed that the internal glycerol level changed in cells exposed to cell wall perturbation.

Keywords

Saccharomyces cerevisiae Heat shock PKC pathway Cell wall stress Glycerol 

Abbreviations

CFW

Calcofluor-white

FG

Filamentous/invasive growth

Glycerol-3-P phosphatase

glycerol-3-phosphate phosphatase

HOG

High osmolarity glycerol

MAP

Mitogen-activated protein

PKC

Protein kinase C

ts

Thermosensitivity

wt

Wild type

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Iwona Wojda
    • 1
  • Jan-Paul Bebelman
    • 2
  • Teresa Jakubowicz
    • 1
  • Marco Siderius
    • 2
  1. 1.Department of Invertebrate Immunology, Institute of BiologyMaria Curie-Sklodowska UniversityLublinPoland
  2. 2.Department of Biochemistry and Molecular Biology, IMBW, Biocentrum AmsterdamVrije UniversiteitAmsterdamThe Netherlands

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