Archives of Microbiology

, Volume 187, Issue 4, pp 313–319

Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520

  • Changming Zhao
  • Yi Luo
  • Chunxu Song
  • Zhongxin Liu
  • Shouwen Chen
  • Ziniu Yu
  • Ming Sun
Original Paper

DOI: 10.1007/s00203-006-0196-3

Cite this article as:
Zhao, C., Luo, Y., Song, C. et al. Arch Microbiol (2007) 187: 313. doi:10.1007/s00203-006-0196-3

Abstract

Zwittermicin A (ZwA) is a novel, broad-spectrum linear aminopolyol antibiotic produced by some Bacillus cereus and Bacillus thuringiensis. However, only part of its biosynthesis cluster has been identified and characterized from B. cereus UW85. To better understand the biosynthesis cluster of ZwA, a bacterial artificial chromosome (BAC) library of B. thuringiensis subsp. kurstaki strain YBT-1520, a ZwA-producing strain, was constructed. Two BAC clones, 1F8 and 5E2, were obtained by PCR, which overlap the known ZwA biosynthesis cluster of B. cereus UW85. This ZwA biosynthesis cluster is at least 38.6 kb and is located on the chromosome, instead of the plasmid. Partial DNA sequencing revealed both BAC clones carry three new ZwA biosynthesis-related genes, zwa6, zwa5A and zwa5B, which were found at the corresponding location of B. cereus UW85. Putative amino acid sequences of these genes shown that ZWA6 is homologous to a typical carbamoyltransferase from Streptomyces avermitilis, while ZWA5A and ZWA5B are homologs of cysteine synthetase and ornithine cyclodeaminase which jointly synthesize 2,3-diaminopropionate in the viomycin biosynthesis pathway, respectively. The identification of these three genes further supports the hypothesized ZwA biosynthesis pathway.

Keywords

Bacillus thuringiensisZwittermicin ABAC library2,3-Diaminopropionate

Abbreviations

A

Adenylation domain

ACP

Acyl carrier domain

BGSC

Bacillus Genetic Stock Center

C

Condensation domain

KS

Ketosynthase

KR

Ketoreductase

NRPS

Nonribosomal peptide synthetase

PCP

Peptidyl carrier protein

PKS

Polyketide synthase

PLP

Pyridoxal 5′-phosphate

ZwA

Zwittermicin A

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Changming Zhao
    • 1
  • Yi Luo
    • 1
  • Chunxu Song
    • 1
  • Zhongxin Liu
    • 1
  • Shouwen Chen
    • 1
  • Ziniu Yu
    • 1
  • Ming Sun
    • 1
  1. 1.College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina