Archives of Microbiology

, Volume 187, Issue 3, pp 207–215

Proteomic analysis of phytopathogenic fungus Botrytis cinerea as a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research

  • Francisco Javier Fernández-Acero
  • Inmaculada Jorge
  • Enrique Calvo
  • Inmaculada Vallejo
  • María Carbú
  • Emilio Camafeita
  • Carlos Garrido
  • Juan Antonio López
  • Jesús Jorrin
  • Jesús Manuel Cantoral
Original Paper

DOI: 10.1007/s00203-006-0188-3

Cite this article as:
Fernández-Acero, F.J., Jorge, I., Calvo, E. et al. Arch Microbiol (2007) 187: 207. doi:10.1007/s00203-006-0188-3

Abstract

Botrytis cinerea is a phytopathogenic fungus causing disease in a substantial number of economically important crops. In an attempt to identify putative fungal virulence factors, the two-dimensional gel electrophoresis (2-DE) protein profile from two B. cinerea strains differing in virulence and toxin production were compared. Protein extracts from fungal mycelium obtained by tissue homogenization were analyzed. The mycelial 2-DE protein profile revealed the existence of qualitative and quantitative differences between the analyzed strains. The lack of genomic data from B. cinerea required the use of peptide fragmentation data from MALDI-TOF/TOF and ESI ion trap for protein identification, resulting in the identification of 27 protein spots. A significant number of spots were identified as malate dehydrogenase (MDH) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The different expression patterns revealed by some of the identified proteins could be ascribed to differences in virulence between strains. Our results indicate that proteomic analysis are becoming an important tool to be used as a starting point for identifying new pathogenicity factors, therapeutic targets and for basic research on this plant pathogen in the postgenomic era.

Keywords

Botrytis cinereaFungal phytopathogenFungal proteomicsVirulence factor

Abbreviations

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

MDH

Malate dehydrogenase

PMF

Peptide mass fingerprinting

2-DE

Two-dimensional gel electrophoresis

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Francisco Javier Fernández-Acero
    • 1
  • Inmaculada Jorge
    • 2
  • Enrique Calvo
    • 3
  • Inmaculada Vallejo
    • 1
  • María Carbú
    • 1
  • Emilio Camafeita
    • 3
  • Carlos Garrido
    • 1
  • Juan Antonio López
    • 3
  • Jesús Jorrin
    • 2
  • Jesús Manuel Cantoral
    • 1
  1. 1.Laboratory of Microbiology, Marine and Environmental Sciences FacultyUniversity of CádizPuerto RealSpain
  2. 2.Agricultural and Plant Biochemistry Research Group, Department of Biochemistry and Molecular BiologyUniversity of CórdobaCórdobaSpain
  3. 3.Proteomics UnitCentro Nacional de Investigaciones Cardiovasculares (CNIC)Tres CantosSpain