Archives of Microbiology

, Volume 186, Issue 2, pp 87–97

Phylogeny of nodulation genes and symbiotic properties of Genista tinctoria bradyrhizobia

  • Michał Kalita
  • Tomasz Stępkowski
  • Barbara Łotocka
  • Wanda Małek
Original Paper

DOI: 10.1007/s00203-006-0124-6

Cite this article as:
Kalita, M., Stępkowski, T., Łotocka, B. et al. Arch Microbiol (2006) 186: 87. doi:10.1007/s00203-006-0124-6

Abstract

Pairwise comparisons of Genista tinctoria (dyer’s weed) rhizobium nodA, nodC, and nodZ gene sequences to those available in databanks revealed their highest sequence identities to nodulation loci of Bradyrhizobium sp. (Lupinus) strains and rhizobia from other genistoid legumes. On phylogenetic trees, genistoid microsymbionts were grouped together in monophyletic clusters, which suggested that their nodulation genes evolved from a common ancestor. G. tinctoria nodulators formed symbioses not only with the native host, but also with other plants of Genisteae tribe such as: Lupinus luteus, Sarothamnus scoparius, and Chamaecytisus ratisbonensis, and they were classified as the genistoid cross-inoculation group. The dyer’s weed root nodules were designated as indeterminate with apical meristem consisting of infected and uninfected cells.

Keywords

Phylogeny of symbiotic genes Genista tinctoria bradyrhizobia Nodule structure Symbiotic properties 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Michał Kalita
    • 1
  • Tomasz Stępkowski
    • 2
  • Barbara Łotocka
    • 3
  • Wanda Małek
    • 1
  1. 1.Department of General MicrobiologyM. Curie-Skłodowska UniversityLublinPoland
  2. 2.Institute of Bioorganic ChemistryPolish Academy of SciencesPoznanPoland
  3. 3.Department of BotanyWarsaw Agricultural UniversityWarsawPoland

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