Archives of Microbiology

, Volume 184, Issue 4, pp 234–248

Wide variation in the cyanobacterial complement of presumptive penicillin-binding proteins

  • Francisco Leganés
  • Amaya Blanco-Rivero
  • Francisca Fernández-Piñas
  • Miguel Redondo
  • Eduardo Fernández-Valiente
  • Qing Fan
  • Sigal Lechno-Yossef
  • C. Peter Wolk
Original Paper

DOI: 10.1007/s00203-005-0046-8

Cite this article as:
Leganés, F., Blanco-Rivero, A., Fernández-Piñas, F. et al. Arch Microbiol (2005) 184: 234. doi:10.1007/s00203-005-0046-8

Abstract

A genomic analysis of putative penicillin-binding proteins (PBPs) that are involved in the synthesis of the peptidoglycan layer of the cell wall and are encoded in 12 cyanobacterial genomes was performed in order to help elucidate the role(s) of these proteins in peptidoglycan synthesis, especially during cyanobacterial cellular differentiation. The analysis suggested that the minimum set of PBPs needed to assemble the peptidoglycan layer in cyanobacteria probably does not exceed one bifunctional transpeptidase–transglycosylase Class A high-molecular-weight PBP; two Class B high-molecular-weight PBPs, one of them probably involved in cellular elongation and the other in septum formation; and one low-molecular-weight PBP. The low-molecular-weight PBPs of all of the cyanobacteria analyzed are putative endopeptidases and are encoded by fewer genes than in Escherichia coli. We show that in Anabaena sp. strain PCC 7120, predicted proteins All2981 and Alr4579, like Alr5101, are Class A high-molecular-weight PBPs that are required for the functional differentiation of aerobically diazotrophic heterocysts, indicating that some members of this class of PBPs are required specifically for cellular developmental processes.

Keywords

CyanobacteriaCell differentiationCell shapeFox genesPenicillin-binding proteinPeptidoglycanHeterocysts

Abbreviations

HMW

High molecular weight

LMW

Low molecular weight

PBP

Penicillin-binding protein

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Francisco Leganés
    • 1
  • Amaya Blanco-Rivero
    • 1
    • 3
  • Francisca Fernández-Piñas
    • 1
  • Miguel Redondo
    • 1
    • 4
  • Eduardo Fernández-Valiente
    • 1
  • Qing Fan
    • 2
    • 5
  • Sigal Lechno-Yossef
    • 2
  • C. Peter Wolk
    • 2
  1. 1.Departamento de Biología, Facultad de CienciasUniversidad Autónoma de MadridMadridSpain
  2. 2.MSU-DOE Plant Research Laboratory and Department of Plant BiologyMichigan State UniversityE. LansingUSA
  3. 3.Department of Plant Physiology, Umeå Plant Sciences CenterUmeå UniversityUmeåSweden
  4. 4.Centre National de la Recherche ScientifiqueInstitut des Sciences du VégétalGif-sur-YvetteFrance
  5. 5.Department of Molecular Pharmacology and Biological ChemistryNorthwestern University Feinberg School of MedicineChicagoUSA