Archives of Microbiology

, Volume 180, Issue 5, pp 367–373

Conjugative plasmid pIP501 undergoes specific deletions after transfer from Lactococcus lactis to Oenococcus oeni

Short Communicaton

DOI: 10.1007/s00203-003-0599-3

Cite this article as:
Zúñiga, M., Pardo, I. & Ferrer, S. Arch Microbiol (2003) 180: 367. doi:10.1007/s00203-003-0599-3

Abstract

Conjugal transfer of plasmids pIP501 and its derivative pVA797 from Lactococcus lactis to Oenococcus oeni was assayed by filter mating. Plasmid pIP501 was transferred to a number of O. oeni strains whereas a single transconjugant of O. oeni M42 was recovered when pVA797 was used. Physical analysis of the transconjugant plasmids revealed that pIP501 and pVA797 underwent extensive deletions in O. oeni that affected the tra region (conjugal transfer) and SegB region (stability). All derivatives showed segregational instability in O. oeni, but were stably maintained in L. lactis. These differences correlated with the different plasmid copy numbers and the extent of deletions within the SegB region.

Keywords

Oenococcus oeni Lactococcus lactis pIP501 pVA797 Conjugation. 

Abbreviations

CAT

Chloramphenicol acetyltransferase

MLS

Macrolides-lincosamides-streptogramin B resistance

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Departament de MicrobiologiaUniversitat de València-BurjassotSpain
  2. 2.Instituto de Agroquímica y Tecnología de los AlimentosBurjassotSpain