Archives of Microbiology

, Volume 178, Issue 6, pp 484–492

Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56

  • Silke Pradella
  • Astrid Hans
  • Cathrin Spröer
  • Hans Reichenbach
  • Klaus Gerth
  • Stefan Beyer
Original Paper

DOI: 10.1007/s00203-002-0479-2

Cite this article as:
Pradella, S., Hans, A., Spröer, C. et al. Arch Microbiol (2002) 178: 484. doi:10.1007/s00203-002-0479-2

Abstract.

In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was found to have various advantageous features, including fast and homogeneous growth in submerged cultures and the ability to complete its morphological differentiation cycle on agar, even when the inoculant originates from a liquid culture. Two groups of secondary metabolites isolated from the culture broth were identified, the polyketides etnangien and chivosazole. The presence of polyketide synthase-encoding genes in the genome of strain So ce56 was demonstrated via PCR. The phenotypic classification was confirmed by comparison of 16S rDNA sequences which showed that S. cellulosum So ce56 clusters within a separate lineage together with S. cellulosum ATCC 25531 and the epothilone producer S. cellulosum So ce90. The genome of S. cellulosum So ce56 belongs to the largest bacterial genomes described so far. It is estimated to be 12.2 Mb in size, by pulsed-field gel electrophoresis. In order to demonstrate that S. cellulosum So ce56 is a convenient strain for molecular biological studies, a genetic manipulation system was developed. Using triparental mating, polyketide synthase-encoding genes were inactivated, leading to chivosazole-negative mutants.

Polyangium (Sorangium) cellulosum Chivosazole Etnangien Triparental mating Genome size Pulsed-field gel electrophoresis Polyketide 16S rDNA 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Silke Pradella
    • 1
  • Astrid Hans
    • 1
  • Cathrin Spröer
    • 2
  • Hans Reichenbach
    • 1
  • Klaus Gerth
    • 1
  • Stefan Beyer
    • 1
  1. 1.German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany
  2. 2.Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany

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