Archives of Microbiology

, Volume 178, Issue 6, pp 443–449

A non-dechlorinating strain of Dehalospirillum multivorans: evidence for a key role of the corrinoid cofactor in the synthesis of an active tetrachloroethene dehalogenase

  • Anke Siebert
  • Anke Neumann
  • Torsten Schubert
  • Gabriele Diekert
Original Paper

DOI: 10.1007/s00203-002-0473-8

Cite this article as:
Siebert, A., Neumann, A., Schubert, T. et al. Arch Microbiol (2002) 178: 443. doi:10.1007/s00203-002-0473-8

Abstract.

A strain of Dehalosprillum multivorans, designated strain N, was isolated from the same source as the formerly described tetrachloroethene (PCE)-dechlorinating D. multivorans, herein after referred to as strain K. Neither growing cells nor cell extracts of strain N were able to dechlorinate PCE. The pceA and pceB genes encoding for the PCE-reductive dehalogenase were detected in cells of strain N; and they were 100% homologous to the corresponding genes of strain K. Since the PCE dehalogenase of D. multivorans strain K contains a corrinoid cofactor, the corrinoids of strain N cells were extracted. Analysis of the corrinoids revealed the absence of the specific corrinoid, which is the cofactor of the PCE dehalogenase of strain K cells. RT-PCR of mRNA indicated that the pceA gene was transcribed in strain N cells to a far lower extent than the pceA gene of strain K under the same experimental conditions. Western blot analysis of crude extracts of strain N showed that, if at all, an insignificant amount of the apoprotein of the PCE dehalogenase was present. The results indicate that the inability of strain N to dechlorinate is due to the absence of the corrinoid cofactor of the enzyme mediating PCE dechlorination.

Corrinoid Dehalorespiration Dehalospirillum multivorans PCE pceA pceB Strain K Strain N Tetrachloroethene Tetrachloroethene reductive dehalogenase 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Anke Siebert
    • 1
  • Anke Neumann
    • 1
  • Torsten Schubert
    • 1
  • Gabriele Diekert
    • 1
  1. 1.Institut für Mikrobiologie, Lehrstuhl für Angewandte und Ökologische Mikrobiologie, FSU Jena, Philosophenweg 12, 07743 Jena, Germany

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