Archives of Microbiology

, Volume 178, Issue 4, pp 301–305

Mesorhizobium amorphae, a rhizobial species that nodulates Amorpha fruticosa, is native to American soils

  • En Wang
  • Antonio M. Rogel
  • Xin Sui
  • Wen Chen
  • Esperanza Martínez-Romero
  • Peter van Berkum
Short Communication

DOI: 10.1007/s00203-002-0448-9

Cite this article as:
Wang, E., Rogel, A.M., Sui, X. et al. Arch Microbiol (2002) 178: 301. doi:10.1007/s00203-002-0448-9

Abstract.

Amorpha fruticosa was inoculated with rhizosphere soil from Iowa, USA, and 140 rhizobia isolated from root nodules were compared with Mesorhizobium amorphae originating from Chinese soils. PCR-RFLP patterns of the 16S rRNA gene from the isolates and from M. amorphae were the same. All isolates had a symbiotic plasmid of the same size with a single nifH gene. DNA:DNA hybridization values, DNA G+C content, and induced Nod factor patterns also were similar. We concluded that the four genotypes distinguished among 53 representative American isolates were M. amorphae. Since A. fruticosa is native to the Americas and is highly specific in its nodulation requirement, M. amorphae probably was transmitted to China.

Amorpha fruticosa Mesorhizobium amorphae Rhizobia Symbiosis Nitrogen fixation Plant-microbe interaction

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • En Wang
    • 1
  • Antonio M. Rogel
    • 2
  • Xin Sui
    • 3
  • Wen Chen
    • 3
  • Esperanza Martínez-Romero
    • 2
  • Peter van Berkum
    • 4
  1. 1.Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, IPN, México D. F., México
  2. 2.Programa de Ecología Microbiana Molecular, Centro de Investigación sobre Fijación de Nitrógeno, UNAM, Cuernavaca, Morelos, México
  3. 3.Department of Microbiology, College of Biology, China Agricultural University, Beijing 100094, People's Republic of China
  4. 4.Soybean Genomics and Improvement Laboratory, Agriculture Research Service, U. S. Department of Agriculture, ARS, HH-4, Bldg010, BARC-West, 10300 Baltimore Blvd., Beltsville, MD 20705, USA