Archives of Microbiology

, Volume 178, Issue 2, pp 131–140

Phylogeny of green sulfur bacteria on the basis of gene sequences of 16S rRNA and of the Fenna-Matthews-Olson protein

  • Boris Alexander
  • Jesper H. Andersen
  • Raymond P. Cox
  • Johannes F. Imhoff
Original Paper

DOI: 10.1007/s00203-002-0432-4

Cite this article as:
Alexander, B., Andersen, J.H., Cox, R.P. et al. Arch Microbiol (2002) 178: 131. doi:10.1007/s00203-002-0432-4

Abstract.

The phylogeny of green sulfur bacteria was studied on the basis of gene sequences of the 16S rRNA and of the Fenna-Matthews-Olson (FMO) protein. Representative and type strains (31 strains total) of most of the known species were analyzed. On the basis of fmoA gene sequences from Chlorobium tepidum ATCC 49652T and Chlorobium limicola DSM 249T available from the EMBL database, primers were constructed that allowed sequence analysis of a major part of the fmoA gene. The largely congruent phylogenetic relationship of sequences of the fmoA gene and of 16S rDNA gives considerable support to the phylogeny of green sulfur bacteria previously suggested on the basis of 16S rDNA sequences. Distinct groups of strains were recognized on the basis of 16S rDNA and FMO sequences and supported by characteristic signature amino acids of FMO. Marine strains formed clusters separate from freshwater strains. The resulting phylogenetic grouping and relationship of the green sulfur bacteria do not correlate with their current taxonomic classification.

Green sulfur bacteria Chlorobiaceae Chlorobium Nucleotide sequences Ribosomal DNA 16S rDNA Phylogeny fmoA 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Boris Alexander
    • 1
  • Jesper H. Andersen
    • 2
  • Raymond P. Cox
    • 2
  • Johannes F. Imhoff
    • 1
  1. 1.Institut für Meereskunde an der Universität Kiel, Düsternbrooker Weg 20, 24105 Kiel, Germany
  2. 2.Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark

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