Archives of Microbiology

, Volume 177, Issue 5, pp 420–426

Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: substrate specificity of the native enzyme and its corrinoid cofactor

  • Anke Neumann
  • Anke Siebert
  • Tina Trescher
  • Simone Reinhardt
  • Gert Wohlfarth
  • Gabriele Diekert
Original Paper

DOI: 10.1007/s00203-002-0409-3

Cite this article as:
Neumann, A., Siebert, A., Trescher, T. et al. Arch Microbiol (2002) 177: 420. doi:10.1007/s00203-002-0409-3

Abstract.

The substrate specificity of the tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans and its corrinoid cofactor were studied. Besides reduced methyl viologen, titanium(III) citrate could serve as electron donor for reductive dehalogenation of tetrachloroethene (PCE) and trichloroethene to cis-1,2-dichloroethene. In addition to chlorinated ethenes, chlorinated propenes were reductively dechlorinated solely by the native enzyme. trans-1,3-Dichloropropene, 1,1,3-trichloropropene and 2,3-dichloropropene were reduced to a mixture of mono-chloropropenes, 1,1-dichloropropene, and 2-chloropropene, respectively. Other halogenated compounds that were rapidly reduced by the enzyme were also dehalogenated abiotically by the heat-inactivated enzyme and by commercially available cyanocobalamin. The rate of this abiotic reaction was dependent on the number and type of halogen substituents and on the type of catalyst. The corrinoid cofactor purified from the tetrachloroethene dehalogenase of D. multivorans exhibited an activity about 50-fold higher than that of cyanocobalamin (vitamin B12) with trichloroacetate as electron acceptor, indicating that the corrinoid cofactor of the PCE dehalogenase is not cyanocobalamin. Corrinoids catalyzed the rapid dehalogenation of trichloroacetic acid. The rate was proportional to the amount of, e.g. cyanocobalamin; therefore, the reductive dehalogenation assay can be used for the sensitive and rapid quantification of this cofactor.

Dehalospirillum multivorans Tetrachloroethene reductive dehalogenase Reductive dehalogenation Chloropropene Cyanocobalamin Vitamin B12 Corrinoid Corrinoid assay Tetrachloroethene Trichloroacetate Alkyl halide 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Anke Neumann
    • 1
  • Anke Siebert
    • 1
  • Tina Trescher
    • 2
  • Simone Reinhardt
    • 2
  • Gert Wohlfarth
    • 2
  • Gabriele Diekert
    • 1
  1. 1.Institut für Mikrobiologie, Lehrstuhl für Angewandte und Ökologische Mikrobiologie, FSU Jena, Philosophenweg 12, 07743 Jena, Germany
  2. 2.Institut für Mikrobiologie, Allmandring 31, Universität Stuttgart, 70569 Stuttgart, Germany

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