Archives of Microbiology

, Volume 177, Issue 4, pp 332–338

Characterization of pMa025, a plasmid from the cyanobacterium Microcystis aeruginosa UV025

  • Margaret M. Wallace
  • David W. Miller
  • Shirley Raps
Original Paper

DOI: 10.1007/s00203-002-0397-3

Cite this article as:
Wallace, M.M., Miller, D.W. & Raps, S. Arch Microbiol (2002) 177: 332. doi:10.1007/s00203-002-0397-3
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Abstract

The characterization of pMa025, a plasmid isolated from the unicellular, toxin-producing cyanobacterium Microcystis aeruginosa UV025, is described. A recombinant plasmid, pMaL [pMa025-pBluescript II SK(–)] was constructed for mapping, sequencing, and development of shuttle vectors capable of transforming both Escherichia coli and M. aeruginosa. pMa025 is 8,018 bp in length and has a G+C content of 62.3 mol%. Nineteen presumptive ORFs, ORF A – ORF S were identified using ATG or GTG as initiation codons. Fifteen different ORFs, ORF a – ORF o were identified using TGA as a degenerate codon for tryptophan. GTG was the start codon in two-thirds of the putative ORFs when TGA was the termination codon. GTG was the start codon in one-third of the putative ORFs when TGA was used as a codon for tryptophan. The deduced amino acid sequence from ORF j (3,114 bp) was significantly similar to that of a putative plasmid replication protein, RepA, from plasmid pUH24 of Synecho coccus sp. strain PCC7942. M. aeruginosa UV027 and E. coli were transformed to carbenicillin resistance with pMaL-D7, a 6.4-kb hybrid plasmid (3.46 kb pMa025, 2.95 kb pBluescript II) generated from the nested deletion strategy. pMaL-D7 will be used as a shuttle vector.

Microcystis aeruginosaPlasmidNucleotidesequence Shuttle vector

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Margaret M. Wallace
    • 1
  • David W. Miller
    • 1
  • Shirley Raps
    • 1
  1. 1.Department of Biological SciencesHunter College, CUNYNew YorkUSA
  2. 2.Department of ScienceJohn Jay College of Criminal JusticeNew YorkUSA