Osteoporosis International

, Volume 23, Issue 4, pp 1245–1253

Teriparatide increases the maturation of circulating osteoblast precursors

  • P. D’Amelio
  • C. Tamone
  • F. Sassi
  • L. D’Amico
  • I. Roato
  • S. Patanè
  • M. Ravazzoli
  • L. Veneziano
  • R. Ferracini
  • G. P. Pescarmona
  • G. C. Isaia
Original Article

DOI: 10.1007/s00198-011-1666-2

Cite this article as:
D’Amelio, P., Tamone, C., Sassi, F. et al. Osteoporos Int (2012) 23: 1245. doi:10.1007/s00198-011-1666-2

Abstract

Summary

This study shows that teriparatide promotes the circulating osteoblast (OB) precursor degree of maturation in patients affected by postmenopausal osteoporosis.

Introduction

Anabolic treatment with teriparatide has proven effective for the therapy of postmenopausal osteoporosis and significantly reduces the risk of non-vertebral fragility fractures. The aim of this study was to investigate the effect of teriparatide on circulating OB precursors.

Methods

We evaluated by flow cytometry and real-time PCR the expression of OBs typical markers in peripheral blood mononuclear cells during treatment with teriparatide plus calcium and vitamin D, raloxifene plus calcium and vitamin D or calcium and vitamin D alone at various time points. Serum bone alkaline phosphatase and osteocalcin (OC) were measured as markers of bone turnover.

Results

Our results show that circulating OB precursors are more numerous and more immature in patients affected by fragility fractures than in osteoporotic patients without fractures. We also show that teriparatide treatment increases the expression of alkaline phosphatase and of OC in OB precursors; thus, it increases their degree of maturation.

Conclusions

We suggest that teriparatide acts as anabolic agents also by promoting the maturation of OB precursors.

Keywords

FracturesOsteoblast precursorsOsteoporosisTeriparatide

Supplementary material

198_2011_1666_MOESM1_ESM.ppt (272 kb)
supplemental Fig. 1Flow cytometry analyses of OB precursors. aDot plot represents PBMCs, monocytes are indicated (R1). bDot plot represents CD 15 positive cells (R2) analyzed on the whole PBMCs population. cDot plot represents monocytes positive for AP and OC, CD15-positive cells (R2) were excluded by the analyses. d Histogram represents the mean fluorescence intensity (MFI) of OC+ cells (gated on monocytes after exclusion of CD 15+ cells). Broken line is the isotype control; unbroken line is the stained sample. (PPT 271 kb)
198_2011_1666_MOESM2_ESM.doc (26 kb)
supplemental Table 1Gene primer sequences. F forward, R reverse. All the genes were quantified by considering signals under 33 Ct. (DOC 26 kb)

Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2011

Authors and Affiliations

  • P. D’Amelio
    • 1
  • C. Tamone
    • 1
  • F. Sassi
    • 1
  • L. D’Amico
    • 2
  • I. Roato
    • 2
  • S. Patanè
    • 2
  • M. Ravazzoli
    • 1
  • L. Veneziano
    • 1
  • R. Ferracini
    • 2
    • 3
  • G. P. Pescarmona
    • 2
    • 4
  • G. C. Isaia
    • 1
  1. 1.Department of Surgical and Medical Disciplines Gerontology SectionUniversity of Torino-ItalyTorinoItaly
  2. 2.CeRMS (Center for Research and Medical Studies)A.O.U. San Giovanni BattistaTorinoItaly
  3. 3.Department of OrthopaedicsA.O.U. San Giovanni BattistaTorinoItaly
  4. 4.Department of GeneticsBiology and Biochemistry-University of TorinoTorinoItaly