Osteoporosis International

, Volume 19, Issue 9, pp 1343–1354

Collagen maturity, glycation induced-pentosidine, and mineralization are increased following 3-year treatment with incadronate in dogs

  • M. Saito
  • S. Mori
  • T. Mashiba
  • S. Komatsubara
  • K. Marumo
Original Article

DOI: 10.1007/s00198-008-0585-3

Cite this article as:
Saito, M., Mori, S., Mashiba, T. et al. Osteoporos Int (2008) 19: 1343. doi:10.1007/s00198-008-0585-3

Abstract

Summary

Collagen cross-linking is a determinant of bone quality. A three-year treatment of bisphosphonate—incadronate disodium—in beagles increased degree of mineralization, collagen maturity, and pentosidine, a compound with advanced glycation end products. The treatment had no effect on the total amount of enzymatic cross-link formation.

Introduction

Collagen cross-linking is a determinant of bone quality. Recently, we reported that long-term treatment with bisphosphonate increased microdamage accumulation. The aim of this study was to clarify the effect of a three-year treatment with bisphosphonate on degree of mineralization and immature and mature enzymatic cross-links and non-enzymatic collagen cross-link, pentosidine, in cortical bone in the same dogs.

Methods

Twenty-nine 1-year-old beagles (15 males, 14 females) were divided into three groups that daily were given vehicle or incadronate at doses of 0.3 or 0.6 mg/kg/day orally for three years. A cortex of a rib was fractionated into low- and high-density portions. The contents of calcium, phosphorus, enzymatic immature and mature cross-links, and the non-enzymatic glycation product pentosidine were determined in each fraction.

Results

Calcium, phosphorus, and pentosidine contents and the ratio of mature to immature cross-links increased significantly with incadronate in a dose-dependent manner, but the total amount of enzymatic cross-links was unchanged. The pentosidine content correlated inversely with cortical activation frequency (p < 0.01).

Conclusion

Long-term suppression of bone remodeling by bisphosphonate increases degree of mineralization, collagen maturity, and non-enzymatic cross-linking.

Keywords

Advanced glycation end products Bisphosphonate Collagen cross-links Mineralization Non-enzymatic glycation Pentosidine 

Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2008

Authors and Affiliations

  • M. Saito
    • 1
  • S. Mori
    • 2
  • T. Mashiba
    • 3
  • S. Komatsubara
    • 3
  • K. Marumo
    • 1
  1. 1.Department of Orthopaedic SurgeryJikei University School of MedicineMinato-kuJapan
  2. 2.Department of Bone and Joint SurgerySeirei Hamamatsu General HospitalHamamatsuJapan
  3. 3.Department of Orthopaedic SurgeryKagawa UniversityKagawaJapan