International Urogynecology Journal

, Volume 13, Issue 2, pp 80–87

Collagen Metabolism and Turnover in Women with Stress Urinary Incontience and Pelvic Prolapse

  • B. H. Chen
  • Y. Wen
  • H. Li
  • M. L. Polan
Original Article

DOI: 10.1007/s001920200020

Cite this article as:
Chen, B., Wen, Y., Li, H. et al. Int Urogynecol J (2002) 13: 80. doi:10.1007/s001920200020

Abstract

The aim of this study was to investigate quantitative mRNA expression of matrix metalloproteinases MMP-1, MMP-2, MMP-9, and their inhibitors, the tissue inhibitors of metalloproteinases TIMP-1, TIMP-2 and TIMP-3, in vaginal wall tissue from women with stress urinary incontinence compared to continent controls. Vaginal wall tissues were obtained from 7 women with stress urinary incontinence/severe pelvic prolapse and 15 continent controls. RNA was then extracted and quantified. Quantitative competitive reverse transcription (QC-RT-PCR) was carried out with oligonucleotide primers to quantify MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3 mRNA expression. Stress continent women demonstrated a significant decrease in TIMP-1 and mRNA expression (P= 0.03). There was no difference in TIMP-2, TIMP-3, MMP-2 or MMP-9 mRNA expression between stress incontinent women and controls. However, MMP-1 mRNA expression was significantly increased (P= 0.05) in the incontinent group and the MMP-1/TIMP-1 ratio (P= 0.04) was consistent with increased collagen degradation in the stress incontinence. Stress incontinent women demonstrated an increase in MMP-1 mRNA expression and a decrease in the inhibitor TIMP-1 mRNA expression. Both these findings are consistent with increased collagen breakdown as a pathologic etiology of incontinence.

Key words:Collagen – Incontinence – Matrix metalloproteinases – Tissue inhibitors of metalloproteinases

Copyright information

© Springer-Verlag London Limited 2002

Authors and Affiliations

  • B. H. Chen
    • 1
  • Y. Wen
    • 1
  • H. Li
    • 1
  • M. L. Polan
    • 1
  1. 1.Stanford University Medical School, Stanford, California; USAUS