International Urogynecology Journal

, Volume 21, Issue 6, pp 705–710

Long-term and short-term effects of simulated birth trauma, cesarean and vaginal delivery on sulfated glycosaminoglycans in the urethra of female rats

  • Claudia Cristina Takano
  • Leonardo Robson Pinheiro Sobreira Bezerra
  • Eliana Viana Monteiro Zucchi
  • Helena Bonciani Nader
  • Marair Gracio Ferreira Sartori
  • Manoel João Batista Castelo Girão
Original Article

DOI: 10.1007/s00192-009-1087-3

Cite this article as:
Takano, C.C., Bezerra, L.R.P.S., Zucchi, E.V.M. et al. Int Urogynecol J (2010) 21: 705. doi:10.1007/s00192-009-1087-3

Abstract

Introduction and hypothesis

This study assesses the effects of simulated birth trauma, vaginal and cesarean delivery on glycosaminoglycans in the connective tissue of female rat urethra.

Methods

One hundred and ten female rats were divided into six groups: A—control, B—vaginal trauma, C—cesarean delivery, D—cesarean delivery followed by vaginal trauma, E—vaginal delivery, and F—female rats on the 20th day of gestation. In each group, half of the animals were sacrificed 4 days after the procedures and half were sacrificed 12 weeks later. Glycosaminoglycans were extracted by proteolysis and analyzed using densitometry after agarose gel electrophoresis.

Results

The examination at 4 days showed larger amounts of glycosaminoglycans in pregnant female rats compared to other groups. The examination at 12 weeks showed larger amounts of glycosaminoglycans in the cesarean delivery group followed by simulated birth trauma.

Conclusions

There is a change in sulfated glycosaminoglycans in female rat urethra during pregnancy and simulated birth trauma.

Keywords

Birth trauma Glycosaminoglycans Pregnancy 

Abbreviations

GAG

Glycosaminoglycans

GAGs

Sulfated glycosaminoglycans

DS

Dermatan sulfate

Copyright information

© The International Urogynecological Association 2010

Authors and Affiliations

  • Claudia Cristina Takano
    • 1
  • Leonardo Robson Pinheiro Sobreira Bezerra
    • 1
  • Eliana Viana Monteiro Zucchi
    • 1
  • Helena Bonciani Nader
    • 2
  • Marair Gracio Ferreira Sartori
    • 1
  • Manoel João Batista Castelo Girão
    • 1
  1. 1.Section of Urogynecology and Pelvic Surgery, Department of GynecologyFederal University of São PauloSão PauloBrazil
  2. 2.Department of BiochemistryFederal University of São PauloSão PauloBrazil