Knee

Knee Surgery, Sports Traumatology, Arthroscopy

, Volume 7, Issue 2, pp 75-80

First online:

Comparison of biochemical characteristics of cultured fibrochondrocytes isolated from the inner and outer regions of human meniscus

  • T. TanakaAffiliated withDepartment of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan Tel.: +81-3-3433-1111, ext. 3441 Fax: +81-3-3459-9114
  • , Katsuyuki FujiiAffiliated withDepartment of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan Tel.: +81-3-3433-1111, ext. 3441 Fax: +81-3-3459-9114
  • , Yoshio KumagaeAffiliated withDepartment of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105 Japan Tel.: +81-3-3433-1111, ext. 3441 Fax: +81-3-3459-9114

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Abstract

In order to evaluate the ability of fibrochondrocytes to synthesize collagen and proteoglycan, human medial meniscal cells were cultured in a monolayer. Meniscal cells were prepared from the two regions (outer 1/3 and inner 2/3) in consideration of the difference in vascular supply, and articular chondrocytes were also obtained from the same knee joint. Regarding total collagen synthesis, regional differences were not found, but age-related differences were found in human medial meniscus. In contrast, proteoglycan synthesis revealed significant regional differences; meniscal cells from the inner 2/3 synthesized a greater amount of proteoglycan. After long-term monolayer culturing, proteoglycan synthesis by meniscal cells decreased in a time-dependent manner, and morphological changes to fibroblast-like cells were found. In the presence of transforming growth factor (TGF)-β, proteoglycan synthesis increased in a dose-dependent manner. These findings suggest that the inner regions of the human meniscus contain cells with a chondrocytic phenotype.

Key words Meniscus Fibrochondrocytes Fibrocartilage Proteoglycan Collagen